Ahomadegbe J.C., Dollet Michel, Gargani Daniel, Riou G..
1990. Characterization of Phytomonas isolates by analysis of the kinetoplast DNA restriction patterns and sequences homology.
In : International congress of parasitology. Baltz T., Walliker D
Autre titre : Caractérisation d'isolats de Phytomonas par analyse des dispositifs de réduction et des homologies de séquence de l'ADN kinétoplastique
Résumé : The kinetoplast DNA (kDNA) was purified from ten #Phytomonas# isolates originating from various countries and recently cultured #in vitro#. Topoisomerase II lesolved the huge kDNA network in free minicircles which were then analyzed by electron microscopy, restriction endonucleases and southern blot hybridization. The sizes of the #Phytomonas# minicircles varied from,1.3 to 2.8 kilobase pairs (kb). Restriction endonuclease analysis showed that they were heterogeneous in base sequences and that heterogeneity could be elevated in certain isolates. However most minicircles from two isolates were homogeneously deaved with a variety of endonucleases, allowing to map the restriction sites. Moreover restriction endonuclease cleavage permitted to separate after electrophoresis maxicircles usually present in low proportion (<5% of the total kDNA). Cross hybridization experiments were performed by Southem blot technique using free minicircles from different isolates as probes. Data show that minicircles from most #Phytomonas# isolates contain homologous DNA sequences ranging from less than 10% to large homology. In conclusion, our data suggest that the restriction cleavage and hybridization techniques could be used to characterize #Phytomonas#.
Mots-clés Agrovoc : Phytomonas, culture in vitro, adn, séquence nucléotidique, microscopie, enzyme de restriction, analyse enzymatique, électrophorèse, variation génétique
Mots-clés complémentaires : Culture de microorganisme
Classification Agris : H20 - Maladies des plantes
Auteurs et affiliations
- Ahomadegbe J.C.
- Dollet Michel
- Gargani Daniel
- Riou G.
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/400812/)
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