Yuan Zhiming, Nielsen Leroux Christina, Pasteur Nicole, Delecluse A., Charles Jean François, Frutos Roger.
1998. Detection of the binary toxin genes of several Bacillus sphaericus strains and their toxicities against susceptible and resistant Culex pipiens.
In : Meetings program and abstracts = [Programme et résumés des rencontres]/
Autre titre : Mise en évidence des gènes binaire de toxine de plusieurs souches de Bacillus sphaericus ainsi que de leur pouvoir toxique contre des moustiques, Culex pipiens, sensibles ou resistants
Résumé : Using synthetic oligonucleotide primers designed on the basis of the binary, toxin gene sequence of 2362, a 1.1 kb DNA fragment has been produced by, PCR and prepared as a probe to detect the toxin genes of several Bacillus sphaericus strains. The 3.5kb Hindlll DNA fragment Of C3-41, IAB881, IAB872 and BS-197 as well as the 4.7 kb HindIll DNA fragment of LPI-G are homologous to the specific probe. All strains could produce the 51.4 and 41.9 kilodalton binary toxins assembled as crystals during their sporulations, which has been proved by SDS-PAGE and Western Blotting. The results of the bioassay showed that C3-41, IAB881, BS-197 land the reference strain 2362 have a high potency, against susceptible Culex pipiens subsp. pipiens and no potency against the resistant mosquito larvae from France(SPHRAE). IAB872 and LPI-G have medium toxicities against the resistant larvae.
Mots-clés Agrovoc : Bacillus sphaericus, gène, séquence nucléotidique, toxine, Culex pipiens
Classification Agris : H10 - Ravageurs des plantes
Auteurs et affiliations
- Yuan Zhiming
- Nielsen Leroux Christina
- Pasteur Nicole
- Delecluse A.
- Charles Jean François
- Frutos Roger, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/401202/)
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