Chaix Gilles, Chantal Isabelle, Poitel Mireille, Razafiarivelo Sylvie, Verhaegen Daniel, Maillard Jean-Charles. 2002. Microsatellite primer amplication by multiplexing: a first application to Eucalyptus grandis. Plant Molecular Biology Reporter, 20 (1), 67a-67e
Résumé : Multiplexing was used to increase the efficiency of microsatellite analysis in Eucalyptus grandis. Primers were chosen based on the range of eucalyptus SSR alleles and their hybridization temperatures. Choice of PCR conditions proved to be the most important factor; concentrations of primers must be adjusted for use in combination. Results show that 7 primers can be analyzed in 2 PCR reactions, one containing 3 and the other containing 4 primers. Multiplexing promises to be a particularly efficient tool for microsatellite diversity analysis in Eucalyptus grandis.
Mots-clés Agrovoc : Eucalyptus grandis, séquence nucléotidique, gène, hybridation d'ADN
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
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Chaix Gilles, CIRAD-FORET-PLANTATIONS (MDG)
ORCID: 0000-0003-2015-0551
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Chantal Isabelle, CIRAD-EMVT-SANTE ANIMALE (FRA) ORCID: 0000-0002-3429-484X
- Poitel Mireille, CIRAD-FORET-PLANTATIONS (FRA)
- Razafiarivelo Sylvie, CENDRADERU (MDG)
- Verhaegen Daniel, CIRAD-FORET-PLANTATIONS (FRA)
- Maillard Jean-Charles, CIRAD-EMVT-SANTE ANIMALE (FRA)
Autres liens de la publication
- Document en bibliothèque
- Localisation du document : BA_BR1961 [(Bibliothèque de Baillarguet)] ; BA_BR2210 [(Bibliothèque de Baillarguet)]
Source : Cirad - Agritrop (https://agritrop.cirad.fr/489853/)
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