Chen Mingsheng, Presting Gernot, Barbazuk W. Brad, Goicoechea Jose Luis, Blackmon Barbara, Fang Guangchen, Kim Hyeran, Frisch David, Yu Yeisoo, Sun Shouhong, Higingbottom Stéphanie, Phimphilai John, Phimphilai Dao, Thurmond Scheen, Li Ping, Liu Jingdong, Hatfield Jamie, Main Dorrie, Farrar Kasey, Henderson Caroline, Barnett Laura, Costa Ravi, Williams Brian, Walser Suzanne, Atkins Michael, Hall Caroline, Budiman Muhammad A., Tomkins Jeffery P., Luo Meizhong, Bancroft Ian, Salse Jérôme, Regad Farid, Mohapatra Trilochan, Singh Nagendra K., Tyagi Akhilesh K., Soderlund Carol, Dean Ralph A., Wing Rod A.. 2002. An integrated physical and genetic map of the rice genome. Plant Cell, 14 : 537-545.
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Résumé : Rice was chosen as a model organism for genome sequencing because of its economic importance, small genome size, and syntenic relationship with other cereal species. We have constructed a bacterial artificial chromosome fingerprint-based physical map of the rice genome to facilitate the whole-genome sequencing of rice. Most of the rice genome (~90.6%) was anchored genetically by overgo hybridization, DNA gel blot hybridization, and in silico anchoring. Genome sequencing data also were integrated into the rice physical map. Comparison of the genetic and physical maps reveals that recombination is suppressed severely in centromeric regions as well as on the short arms of chromosomes 4 and 10. This integrated high-resolution physical map of the rice genome will greatly facilitate whole-genome sequencing by helping to identify a minimum tiling path of clones to sequence. Furthermore, the physical map will aid map-based cloning of agronomically important genes and will provide an important tool for the comparative analysis of grass genomes.
Mots-clés Agrovoc : Oryza, carte génétique, génome, adn
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Chen Mingsheng
- Presting Gernot
- Barbazuk W. Brad
- Goicoechea Jose Luis
- Blackmon Barbara
- Fang Guangchen
- Kim Hyeran
- Frisch David
- Yu Yeisoo
- Sun Shouhong
- Higingbottom Stéphanie
- Phimphilai John
- Phimphilai Dao
- Thurmond Scheen
- Li Ping
- Liu Jingdong
- Hatfield Jamie
- Main Dorrie
- Farrar Kasey
- Henderson Caroline
- Barnett Laura
- Costa Ravi
- Williams Brian
- Walser Suzanne
- Atkins Michael
- Hall Caroline
- Budiman Muhammad A.
- Tomkins Jeffery P.
- Luo Meizhong
- Bancroft Ian
- Salse Jérôme
- Regad Farid
- Mohapatra Trilochan
- Singh Nagendra K.
- Tyagi Akhilesh K.
- Soderlund Carol
- Dean Ralph A.
- Wing Rod A.
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/511151/)
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