Identification and fine mapping of Pi33, the rice resistance gene corresponding to the magnaporthe grisea avirulence gene ACE1

Berruyer Romain, Adreit Henri, Milazzo Joëlle, Gaillard Sylvain, Berger Angélique, Dioh Waly, Lebrun Marc-Henri, Tharreau Didier. 2003. Identification and fine mapping of Pi33, the rice resistance gene corresponding to the magnaporthe grisea avirulence gene ACE1. Theoretical and Applied Genetics, 107 : 1139-1147.

Résumé : Rice blast disease is a major constraint for rice breeding. Nevertheless, the genetic basis of resistance remains poorly understood for most rice varieties, and new resistance genes remain to be identified. We identified the resistance gene corresponding to the cloned avirulence gene ACE1 using pairs of isogenic strains of Magnaporthe grisea differing only by their ACE1 allele. This resistance gene was mapped on the short arm of rice chromosome 8 using progenies from the crosses IR64 (resistant) x Azucena (susceptible) and Azucena x Bala (resistant). The isogenic strains also permitted the detection of this resistance gene in several rice varieties, including the differential isogenic line C101LAC. Allelism tests permitted us to distinguish this gene from two other resistance genes [Pi11 and Pi-29(t)] that are present on the short arm of chromosome 8. Segregation analysis in F2 populations was in agreement with the existence of a single dominant gene, designated as Pi33. Finally, Pi33 was finely mapped between two molecular markers of the rice genetic map that are separated by a distance of 1.6 cM. Detection of Pi33 in different semi-dwarf indica varieties indicated that this gene could originate from either one or a few varieties.

Mots-clés Agrovoc : Oryza sativa, résistance aux maladies, gène, séquence nucléotidique, carte génétique, Magnaporthe grisea

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

Auteurs et affiliations

• Berruyer Romain, CIRAD-CA-CALIM (FRA)
• Adreit Henri, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
• Milazzo Joëlle, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
• Gaillard Sylvain, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
• Berger Angélique, CIRAD-CP-COCOTIER (FRA)
• Dioh Waly, IGM [Institut de génétique et microbiologie] (FRA)
• Lebrun Marc-Henri, CNRS (FRA)
• Tharreau Didier, CIRAD-CA-CALIM (FRA) ORCID: 0000-0003-3961-6120

Source : Cirad - Agritrop (https://agritrop.cirad.fr/519084/)

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