Isolation and characterization of polymorphic microsatellite markers in Zabrotes subfasciatus Boheman (Coleoptera: Bruchidae)

Aebi A., Shani T., Butcher R.D.J., Alvarez N., Risterucci Ange-Marie, Benrey B.. 2004. Isolation and characterization of polymorphic microsatellite markers in Zabrotes subfasciatus Boheman (Coleoptera: Bruchidae). Molecular Ecology Notes, 4 (4) : 752-754.

Résumé : Bruchid beetles of the genus Zabrotes (Coleoptera: Bruchidae) are important world wide pests of legume seeds. Samples of Zabrotes subfasciatus and Z. sylvestris were collected from seeds of two Phaseolus species throughout Mexico to assess the role of host plant variation and plant domestication on the evolution of host use in this species. For this purpose six polymorphic microsatellite loci were isolated and characterized for the bruchid Z. subfasciatus. Cross-species amplification tests were performed on Z. sylvestris and revealed that three loci amplified successfully and were polymorphic in this closely related species.

Mots-clés Agrovoc : Zabrotes subfasciatus, Phaseolus, Bruchidae, domestication, variation génétique, polymorphisme génétique, marqueur génétique, microsatellite, séquence nucléotidique, génétique des populations

Mots-clés géographiques Agrovoc : Mexique

Classification Agris : F30 - Génétique et amélioration des plantes
H10 - Ravageurs des plantes

Auteurs et affiliations

• Aebi A., Université de Neuchâtel (CHE)
• Shani T., Université de Neuchâtel (CHE)
• Butcher R.D.J., Bath University (GBR)
• Alvarez N., Université de Neuchâtel (CHE)
• Risterucci Ange-Marie, CIRAD-AMIS-BIOTROP (FRA)
• Benrey B., Université de Neuchâtel (CHE)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/523185/)

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