Enhancing gene targeting efficiency in higher plants : rice is on the move

Cotsaftis Olivier, Guiderdoni Emmanuel. 2005. Enhancing gene targeting efficiency in higher plants : rice is on the move. Transgenic Research, 14 (1) : 1-14.

Résumé : Meeting the challenge of routine gene targeting (GT) in higher plants is of crucial interest to researchers and plant breeders who are currently in need of a powerful tool to specifically modify a given locus in a genome. Higher plants have long been considered the last lineage resistant to targeting technology. However, a recent report described an efficient method of T-DNA-mediated targeted disruption of a non-selectable locus in rice [Terada et al., Nat Biotechnol 20: 1030-1034 (2002)]. Though this study was an obvious breakthrough, further improvement of GT frequencies may derive from a better understanding of the natural mechanisms that control homologous recombination (HR) processes. In this review, we will focus on what is known about HR and the factors which may hamper the development of routine GT by HR in higher plants. We will also present the current strategies envisaged to overcome these limitations, such as expression of recombination proteins and refinements in the design of the transformation vector.

Mots-clés Agrovoc : Oryza sativa, génie génétique, recombinaison, gène, protéine végétale

Mots-clés complémentaires : Recombinaison homologue, Ciblage de gènes

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Cotsaftis Olivier, CIRAD-AMIS-BIOTROP (FRA)
• Guiderdoni Emmanuel, CIRAD-AMIS-BIOTROP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/525260/)

Métriques

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