Novel vip3-related protein from Bacillus thuringiensis

Rang Cécile, Gil Patricia, Neisner Nathalie, Van Rie Jeroen, Frutos Roger. 2005. Novel vip3-related protein from Bacillus thuringiensis. Applied and Environmental Microbiology, 71 (10) : 6276-6281.

Résumé : A novel vip3-related gene was identified in Bacillus thuringiensis. This novel gene is 2,406 bp long and codes for a 91-kDa protein (801 amino acids). This novel protein exhibits between 61 and 62% similarity with Vip3A proteins and is designated Vip3Ba1. Vip3Ba1 has several specific features. Differences between Vip3Ba1 and the Vip3A proteins are spread throughout the sequence but are more frequent in the C-terminal part from amino acid 456 onward. The regions containing the two proteolytic processing sites, which are highly conserved among the Vip3A toxins, are markedly different in Vip3Ba1. The pattern DCCEE (Asp Cys Cys Glu Glu) is repeated four times between position 463 and 483 in Vip3Ba1, generating the sequence 463-DCCEEDCCEE DCCEEDCCEE-483. This sequence, which is rich in negatively charged amino acids, also contains 73% of the cysteines present in Vip3Ba1. This repeated sequence is not present in Vip3A proteins. The Vip3Ba1 protein was produced in Escherichia coli and tested against Ostrinia nubilalis and Plutella xylostella, and it generated significant growth delays but had no larvicidal effect, indicating that its host range might be different than that of Vip3A proteins.

Mots-clés Agrovoc : Bacillus thuringiensis, pesticide bactérien, génie génétique, séquence nucléotidique, identification

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Rang Cécile, Bayer (BEL)
• Gil Patricia, CIRAD-EMVT-UPR Contrôle des maladies (FRA) ORCID: 0000-0001-7984-7340
• Neisner Nathalie, Bayer (BEL)
• Van Rie Jeroen, Bayer (BEL)
• Frutos Roger, CIRAD-EMVT-UMR TRYPANOSOMES (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/534189/)

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