Enhanced solvent extraction of polar lipids associated with rubber particles for Hevea brasiliensis

Bonfils Frédéric, Ehabe Ejolle Eugène, Aymard Christian, Vaysse Laurent, Sainte-Beuve Jérôme. 2007. Enhanced solvent extraction of polar lipids associated with rubber particles for Hevea brasiliensis. Phytochemical Analysis, 18 (2) : 103-108.

Résumé : Abstract: Biochemical studies of lipids bound to rubber particles have been complicated due to the solubility of polyisoprene chains in most extracting solvents and the rather delicate nature of polar lipids that arc often denatured when traditional solvent extraction techniques are employed. In this paper, we describe a traditional technique and accompanying solvents that permit optimal extraction of rubber particle bound lipids. The technique, which is validated after characterizing the lipid extracts by elemental analysis, silica column adsorption and thin layer chromatography, appeared more suitable for extracting total lipids with optimal glycolipid and phospholipid contents. This technique is proposed as an alternative to traditional extraction methods used for solid natural rubber as it offers advantages with respect to ease of application, extract quality, extraction yields and reproducibility. Copyright. © 2007 John Wiley & Sons, Ltd.

Mots-clés Agrovoc : caoutchouc, Hevea brasiliensis, extraction par solvant, lipide, teneur en lipides, méthanol, acétone, chloroforme, Immersion, glycolipide, phosphatide

Mots-clés complémentaires : Caoutchouc naturel, Phospholipide

Classification Agris : Q60 - Traitement des produits agricoles non alimentaires

Champ stratégique Cirad : Hors axes (2005-2013)

Auteurs et affiliations

• Bonfils Frédéric, CIRAD-CP-UPR Qualité produits pérennes (FRA) ORCID: 0000-0001-7016-4770
• Ehabe Ejolle Eugène, IRAD (CMR)
• Aymard Christian
• Vaysse Laurent, CIRAD-CP-UPR Qualité produits pérennes (THA) ORCID: 0000-0003-3264-6116
• Sainte-Beuve Jérôme, CIRAD-CP-DIR (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/537797/)

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