In silico and in vitro analysis of the isoprenoid pathway in coffee

Tiski Iris, Perreira Luiz Filipe Protasio, Vieira Luiz Gonzaga Esteves, Takahashi E.K., Ferreira Lucia Pires, Marraccini Pierre, Pot David, Leroy Thierry, Benassi Marta T., Dias Rafael Carlos Eloy. 2007. In silico and in vitro analysis of the isoprenoid pathway in coffee. In : 21st International Conference on Coffee Science, Montpellier (France), 11th - 15th September 2006 = 21ème Colloque scientifique international sur le café ; 21. Internationales Wissenshaftliches Kolloquium über Kaffee ; 21e Coloquio Cientifico Internacional sobre el Café. ASIC. Montpellier : ASIC, 745-748. ISBN 2-900212-20-0 Colloque Scientifique International sur le Café. 21, Montpellier, France, 11 Septembre 2006/15 Septembre 2006.

Résumé : The most important lipids in coffee, the diterpenes khaweol and cafestol, are originated from the isoprenoid pathway. Despite their diversity in functions and structures, all isoprenoids derive from the common-five carbon building unit isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). In higher plants, there are two independent pathways located in the cytosol (mevalonic acid or MVA pathway) and in the plastids (methylerythritol phosphate - MEP - or non mevalonic pathway). Throughout the data mining of the Brazilian Coffee Genome Project we studied the genes that code for the enzymes 3-hydroxy-3-methyglutaryl-CoA reductase (HMGR) and mevalonate diphosphate decarboxylase (MPDC) for the MVA pathway and 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) and isopentenyl diphosphate/dimethylallyl diphosphate synthase (IDS) for the MEP pathway.

Mots-clés Agrovoc : Coffea, fève de café, isoprénoïde, diterpénoïde, métabolisme des lipides, gène, activité enzymatique, oxydoréductase, lyase

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale

Auteurs et affiliations

• Tiski Iris, IAPAR (BRA)
• Perreira Luiz Filipe Protasio, IAPAR (BRA)
• Vieira Luiz Gonzaga Esteves, IAPAR (BRA)
• Takahashi E.K., IAPAR (BRA)
• Ferreira Lucia Pires, IAPAR (BRA)
• Marraccini Pierre, CIRAD-AMIS-UMR PIA (BRA) ORCID: 0000-0001-7637-6811
• Pot David, CIRAD-AMIS-UMR PIA (BRA) ORCID: 0000-0001-6144-8448
• Leroy Thierry, CIRAD-AMIS-UMR PIA (FRA)
• Benassi Marta T., UEL (BRA)
• Dias Rafael Carlos Eloy, UEL (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/540078/)

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