Coffea spp. and Coffea canephora diversity evaluated with microsatellites and SNPs. Lessons from comparative analysis

Leroy Thierry, Cubry Philippe, Durand Noël, Dufour Magali, De Bellis Fabien, Jourdan Isabelle, Vieira Luiz Gonzaga Esteves, Musoli Chungason Pascal, Aluka P., Legnaté Hyacinthe, Marraccini Pierre, Pot David. 2007. Coffea spp. and Coffea canephora diversity evaluated with microsatellites and SNPs. Lessons from comparative analysis. In : 21st International Conference on Coffee Science, Montpellier (France), 11th - 15th September 2006 = 21ème Colloque scientifique international sur le café ; 21. Internationales Wissenshaftliches Kolloquium über Kaffee ; 21e Coloquio Cientifico Internacional sobre el Café. ASIC. Montpellier : ASIC, 827-834. ISBN 2-900212-20-0 Colloque Scientifique International sur le Café. 21, Montpellier, France, 11 Septembre 2006/15 Septembre 2006.

Résumé : Coffea genus includes more than 80 taxa, representing a high genotypic and phenotypic diversity. Our study leads to an evaluation of diversity, based on two types of markers, microsatellites (SSR) and Single Nucleotide Polymorphisms. Coffea diversity was evaluated at two levels: 20 species of the genus were analysed whereas a wider sampling was performed for Coffea canephora. 60 SSR markers were used; they provided new information concerning Coffea genus structuration and phylogenetic relationships between species. At the interspecific level, results are consistent with the geographical distribution of species. Within C. canephora, the knowledge of the genetic diversity of wild and cultivated populations is important as preliminary information for genetic improvement. Results obtained are consistent with previous studies. Furthermore, a new group including the Ugandan genotypes was identified, constituting the most eastern located independent genetic group, with a level of diversity comparable to Congolese genotypes. Analyses on SNPs diversity have also been performed for some specific genes implicated in sucrose and diterpen metabolisms. Results give complementary data on diversity, and indicate if these genes were subjected to natural of artificial selection, indicating the best putative candidate genes. Consequences for the identification of tools for Marker Assisted Selection are discussed.

Mots-clés Agrovoc : Coffea, Coffea canephora, variation génétique, microsatellite, phylogénie, marqueur génétique, génotype, polymorphisme génétique, séquence nucléotidique, carte génétique, métabolisme des glucides, diterpénoïde, saccharose

Mots-clés géographiques Agrovoc : Ouganda, Congo

Classification Agris : F70 - Taxonomie végétale et phytogéographie
F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale

Auteurs et affiliations

• Leroy Thierry, CIRAD-AMIS-UMR PIA (FRA)
• Cubry Philippe, CIRAD-AMIS-UMR PIA (FRA)
• Durand Noël, CIRAD-CP-UPR Qualité produits pérennes (FRA) ORCID: 0000-0003-1627-6848
• Dufour Magali, CIRAD-AMIS-UMR PIA (FRA)
• De Bellis Fabien, CIRAD-AMIS-UMR PIA (FRA) ORCID: 0000-0001-7070-7691
• Jourdan Isabelle, CIRAD-AMIS-UMR PIA (FRA)
• Vieira Luiz Gonzaga Esteves, IAPAR (BRA)
• Musoli Chungason Pascal, CORI (UGA)
• Aluka P., CORI (UGA)
• Legnaté Hyacinthe, CNRA (CIV)
• Marraccini Pierre, CIRAD-AMIS-UMR PIA (BRA) ORCID: 0000-0001-7637-6811
• Pot David, CIRAD-AMIS-UMR PIA (BRA) ORCID: 0000-0001-6144-8448

Source : Cirad - Agritrop (https://agritrop.cirad.fr/540085/)

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