Molecular identification of Western European species of obsoletus complex (Diptera: Ceratopogonidae) by an internal transcribed spacer-1 rDNA multiplex polymerase chain reaction assay

Mathieu Bruno, Perrin Aurélie, Baldet Thierry, Delecolle Jean Claude, Albina Emmanuel, Cetre-Sossah Catherine. 2007. Molecular identification of Western European species of obsoletus complex (Diptera: Ceratopogonidae) by an internal transcribed spacer-1 rDNA multiplex polymerase chain reaction assay. Journal of Medical Entomology, 44 (6) : 1019-1025.

https://doi.org/10.1093/jmedent/44.6.1019

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Résumé : In southern Europe, orbiviral diseases such as bluetongue (BT) have been assumed to have been largely transmitted by the classical Afro-Asian vector Culicoides imicola Kieffer (Diptera: Ceratopogonidae). Recent outbreaks have occurred in regions where C. imicola is normally absent, supporting the theory that other species belonging to the Obsoletus or Pulicaris complexes may play a role in BT virus transmission. Investigations of the ecology of the species within the former group are hampered by females of member species being extremely difficult to separate by classical morphology. To allow straightforward separation of these species in France, a multiplex polymerase chain reaction-based on internal transcribed spacer (ITS)-1 rDNA was developed to distinguish between Culicoides chiopterus Meigen, Culicoides dewulfi Goetghebuer, Culicoides montanus Shakirjanova, Culicoides obsoletus Meigen, and Culicoides scoticus Downes & Kettle. This tool will be useful in defining both the vector role and larval biotopes of these species in Europe.

Mots-clés Agrovoc : Ceratopogonidae, phylogénie, Culicoides, transmission des maladies, vecteur de maladie, identification, PCR, génétique mitochondriale, séquence nucléotidique, fièvre catarrhale ovine

Mots-clés complémentaires : Culicoides obseletus, Culicoides scotilus, Culicoides dewulfi, Culicoides montanus, Culicoides imicola

Classification Agris : L72 - Organismes nuisibles des animaux
L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

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