Statistical optimization of [alpha]-amylase production by probiotic Lactobacillus plantarum MTCC 1407 in submerged fermentation

Panda Smita H., Swain Manas R., Kar Shaktimay, Ray Ramesh C., Montet Didier. 2008. Statistical optimization of [alpha]-amylase production by probiotic Lactobacillus plantarum MTCC 1407 in submerged fermentation. Polish Journal of Microbiology, 57 (2) : 149-155.

Résumé : Production and purification of [alpha]-amylase by probiotic Lactobacillus plantarum MTCC 1407 has been investigated under submerged fermentation using Mann Rogassa Sharpe medium containing (1%) soluble starch in lieu of glucose (2%) as carbon source. Response Surface Methodology was used to evaluate the effect of main variables, i.e. incubation period, pH and temperature on enzyme production. A full factorial Central Composite Design was applied to study these main factors that affected a-amylase production. The experimental results showed that the optimum incubation period, pH and temperature were 36 h, 7.0 and 35°C, respectively. The purified enzyme (by ammonium sulphate precipitation) had a molecular mass of 75 450 Da in SDS-PAGE.

Mots-clés Agrovoc : Lactobacillus plantarum, fermentation, alpha amylase, probiotique

Classification Agris : Q02 - Traitement et conservation des produits alimentaires

Champ stratégique Cirad : Axe 3 (2005-2013) - Alimentation accessible et de qualité

Auteurs et affiliations

• Panda Smita H., CTCRI (IND)
• Swain Manas R., CTCRI (IND)
• Kar Shaktimay, CTCRI (IND)
• Ray Ramesh C., CTCRI (IND)
• Montet Didier, CIRAD-PERSYST-UMR Qualisud (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/544885/)

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