Application of PCR-DGGE in determining food origin : cases studies of fish and fruits

Montet Didier, Le Nguyen Doan Duy, El Sheikha Aly, Condur Ana, Metayer Isabelle, Loiseau Gérard. 2008. Application of PCR-DGGE in determining food origin : cases studies of fish and fruits. In : Greening the food chain, 3 and 4. Includes papers from the workshops : 3. Traceability: Tracking and tracing in the food chain 4. Supplying sustainable and innovative food and drink solutions. Eds by Richard Glass, Wayne Martindale. Wellesbourne : AAB, 11-22. (Aspects of applied biology, 87) Workshop on Traceability : Tacking and Tracing in the Food Chain. 3, York, Royaume-Uni, 15 Novembre 2008.

Résumé : The determination of geographical origin is a demand of the traceability system of import-export food products. For this purpose, molecular techniques employing 16S, 26S rDNA profiles generated by PCR-DGGE (polymerase chain reaction - denaturing gradient gel electrophoresis) were used to detect the variation in microbial community (bacteria, yeast) structures of Pangasius fish from Viet Nam harvested in different aquaculture farms and during different seasons and two fruits Physalis from Egypt and mandarins from Spain and Morocco. In Physalis case, ecology of yeasts was also studied for the same purpose. Bacterial band profiles of fish and mandarins, and yeast band profiles of Physalis were specific for each location and could be used as a bar code to certify the origin of fish and fruits. This method is a new traceability tool which provides food with a unique biological bar code and makes it possible to trace back the food to their original location.

Mots-clés Agrovoc : Pangasius hypophthalmus, mandarine, Physalis ixocarpa, provenance, PCR, électrophorèse, analyse qualitative, flore microbienne

Mots-clés géographiques Agrovoc : Languedoc-Roussillon, Égypte, Viet Nam, France

Classification Agris : E70 - Commerce, commercialisation et distribution
U30 - Méthodes de recherche

Auteurs et affiliations

• Montet Didier, CIRAD-PERSYST-UMR Qualisud (FRA)
• Le Nguyen Doan Duy, Can Tho University (VNM)
• El Sheikha Aly, CIRAD-PERSYST-UMR Qualisud (FRA)
• Condur Ana
• Metayer Isabelle, CIRAD-PERSYST-UMR Qualisud (FRA)
• Loiseau Gérard, CIRAD-PERSYST-UMR Qualisud (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/548193/)

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