Dhuique-Mayer Claudie, Amiot-Carlin M.J..
2009. Bêta-Cryptoxanthin from Citrus Juices: Bioaccessibility and uptake by caco-2 cell culture model.
In : Proceedings of the second international symposium on human health effects of fruits and vegetables, FAVHEALTH 2007, October 9-12, 2007, Houston, USA. Patil B. (ed.), Murano P. (ed.), Amiot-Carlin M.J. (ed.). ISHS
Résumé : Bêta-cryptoxanthin ([bêta]-CX), belonging to the xanthophyll sub-class of carotenoids, is mainly found in citrus fruit in both free and ester forms. Due to the lack of knowledge on the intestinal uptake of [bêta]-CX, especially the ester forms, our purpose was to study the bioaccessibility of carotenoids from citrus juice. Carotenoid uptake was evaluated by incorporating carotenoid-rich micelles (from the in vitro digestion) or synthetic micelles (made from synthetic lipids and carotenoids purified from citrus juice) to human intestinal cells (Caco-2 TC7 clone). The uptake efficiency of [bêta]-CX esters was compared to free [bêta]-CX. Our results suggested that esters were partially hydrolyzed during the in vitro digestion. The bioaccessibility of free [bêta]-CX exceeded that of [bêta]-carotene and [bêta]-CX esters. These results showed a preferential uptake by Caco-2 cells of [bêta]-carotene and free [bêta]-CX compared to the two 'esters for all types of micelles tested. In the case of micelles from in vitro digestion, the uptake of [bêta]-carotene, free [bêta]-CX and [bêta]-CX esters by Caco-2 cells was 14.3 ± 1.8; 3.9 ± 0.9; 0.7 ± 0.08 The present study provides the first report on assessment of [bêta]-CX (free and esters) uptake by differentiated Caco-2 cells.
Mots-clés Agrovoc : jus de fruits, Citrus
Classification Agris : Q04 - Composition des produits alimentaires
Auteurs et affiliations
- Dhuique-Mayer Claudie, CIRAD-PERSYST-UMR Qualisud (FRA)
- Amiot-Carlin M.J., INRA (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/552751/)
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