Sequencing the coffee genome (W152)

Wincker Patrick, Albert Victor A., Andrade Alan A., Argout Xavier, Bertrand Benoît, De Kochko Alexandre, Giuliano Giovanni, Graziosi Giorgio, Henry Robert, Jayarama Jayarama, Lashermes Philippe, Ming Ray, Nagai Chifumi, Rounsley Steve, Sankoff David. 2011. Sequencing the coffee genome (W152). In : Abstracts of Plant and Animal Genomes XIXth Conference, San Diego, CA (USA), January 15-19, 2011. s.l. : s.n., Résumé Plant and Animal Genomes Conference. 19, San Diego, États-Unis, 15 Janvier 2011/19 Janvier 2011.

Résumé : Commercial coffee production relies mainly on two closely related species: Coffea arabica and C. canephora, which account respectively for 70 and 30% of the coffee production. All coffee species are diploid (2n=2x=22) and generally selfincompatible, except for C. arabica which is the only tetraploid (2n=4x=44) and self-fertile. Molecular analyses (Lashermes et al. 1999) have indicated that C. arabica is a recent allotetraploid (CE genome) formed by hybridisation between two related diploid species: C. canephora (C genome) and C. eugenioides (E genome). In spite of the close relationship between the two constitutive sub-genomes, C. arabica displays diploid-like meiotic behavior with bivalent formation (Krug and Mendes 1940, Lashermes et al. 2000). The genomes of coffee species (Cros et al. 1995; Noirot et al. 2003) appear to be of rather low size (i. e. about 660, 710 and 1300 Mb for C. eugenioides, C. canephora and C. arabica, respectively). Several institutes are combining their scientific resources and expertise to sequence, assemble, and annotate the entire genome of C. canephora. The C. canephora genome consists of 11 chromosomes, is about 710 Mb in size, and is being sequenced de novo with deep coverage using 454 paired-end and single reads, and 50x coverage with Illumina GAIIx data to obtain a reference genome for Coffea. The overall sequencing strategy as well as progress of the project will be described.

Mots-clés Agrovoc : Coffea arabica, Coffea canephora, génome

Mots-clés complémentaires : Séquencage

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Wincker Patrick, Institut de génomique (FRA)
• Albert Victor A., University at Buffalo (USA)
• Andrade Alan A., EMBRAPA (BRA)
• Argout Xavier, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-0100-5511
• Bertrand Benoît, CIRAD-BIOS-UMR RPB (FRA) ORCID: 0000-0003-1969-3479
• De Kochko Alexandre, IRD (FRA)
• Giuliano Giovanni, ENEA (ITA)
• Graziosi Giorgio, Universita Degli Studi di Trieste (ITA)
• Henry Robert, University of Queensland (AUS)
• Jayarama Jayarama, CCRI (IND)
• Lashermes Philippe, IRD (FRA)
• Ming Ray, University of Illinois (USA)
• Nagai Chifumi, HARC (USA)
• Rounsley Steve, Dow Agrosciences (USA)
• Sankoff David, University of Ottawa (CAN)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/560006/)

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