Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso

Tiendrebeogo Fidèle, Lefeuvre Pierre, Hoareau Murielle, Villemot Julie, Konaté Gnissa, Traoré Alfred S., Barro Nicolas, Traore Valentin S.E., Reynaud Bernard, Traoré Oumar, Lett Jean-Michel. 2010. Molecular diversity of cotton leaf curl Gezira virus isolates and their satellite DNAs associated with okra leaf curl disease in Burkina Faso. Virology Journal, 7, 10 p.

https://doi.org/10.1186/1743-422X-7-48

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Quartile : Q2, Sujet : VIROLOGY

Résumé : Okra leaf curl disease (OLCD) is a major constraint on okra (Abelmoschus esculentus) production and is widespread in Africa. Using a large number of samples representative of the major growing regions in Burkina Faso (BF), we show that the disease is associated with a monopartite begomovirus and satellite DNA complexes. Twenty-three complete genomic sequences of Cotton leaf curl Gezira virus (CLCuGV) isolates associated with OLCD, sharing 95 to 99% nucleotide identity, were cloned and sequenced. Six betasatellite and four alphasatellite (DNA-1) molecules were also characterized. The six isolates of betasatellite associated with CLCuGV isolates correspond to Cotton leaf curl Gezira betasatellite (CLCuGB) (88 to 98% nucleotide identity). One isolate of alphasatellite is a variant of Cotton leaf curl Gezira alphasatellite (CLCuGA) (89% nucleotide identity), whereas the three others isolates appear to correspond to a new species of alphasatellite (CLCuGA most similar sequence present 52 to 60% nucleotide identity), provisionally named Okra leaf curl Burkina Faso alphasatellite (OLCBFA). Recombination analysis of the viruses demonstrated the interspecies recombinant origin of all CLCuGV isolates, with parents being close to Hollyhock leaf crumple virus (AY036009) and Tomato leaf curl Diana virus (AM701765). Combined with the presence of satellites DNA, these results highlight the complexity of begomoviruses associated with OLCD.

Mots-clés Agrovoc : Abelmoschus esculentus, virus des végétaux, espèce nouvelle, phylogénie, adn, séquence d'adn, recombinaison, taxonomie, virologie, begomovirus

Mots-clés géographiques Agrovoc : Burkina Faso

Mots-clés complémentaires : Cotton leaf curl Gezira virus, Okra leaf curl disease

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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