A genetic map of pineapple (Ananas comosus (L.) Merr.) including SCAR, CAPS, SSR and EST-SSR markers

Carlier Jorge Dias, Sousa Nelson Horta, Santo Tatiana Espirito, Coppens D'Eeckenbrugge Géo, Leitão José Manuel. 2012. A genetic map of pineapple (Ananas comosus (L.) Merr.) including SCAR, CAPS, SSR and EST-SSR markers. Molecular Breeding, 29 (1) : 245-260.

Quartile : Q1, Sujet : HORTICULTURE / Quartile : Q1, Sujet : AGRONOMY / Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q2, Sujet : GENETICS & HEREDITY

Résumé : Despite the paramount importance of pineapple (Ananas comosus L.) in world production and trade of tropical fruits, the genomics of this crop is still lagging behind that of other tropical fruit crops such as banana or papaya. A genetic map of pineapple was constructed using an F2 segregating population obtained from a single selfed F1 plant of a cross A. comosus var. comosus (cv. Rondon, clone BR 50) × A. comosus var. bracteatus (Branco do mato, clone BR 20). Multiple randomly amplified markers (RAPD, ISSR and AFLP) were brought together with SSR and EST-SSR markers identified among sequences uploaded to public databases and with sequence-specific markers (SCAR, SSR and CAPS) derived from random amplified markers. Sixty-three randomly amplified markers (RAPD, ISSR and AFLP) were selected and cloned, resulting in 71 sequences which were used to generate sequence-specific SCAR and CAPS markers. The present map includes 492 DNA markers: 57 RAPD, 22 ISSR, 348 AFLP, 20 SSR, 12 EST-SSR, 25 SCARs, 8 CAPS, and the morphological trait locus "piping", gathered into 33 linkage groups that integrate markers inherited from both botanical varieties, four linkage groups with markers only from var. comosus and three linkage groups with markers exclusively from var. bracteatus. The relatively higher mapping efficiency of sequence-specific markers derived from randomly amplified markers (50.7%) versus SSR (31.4%) and EST-SSR (28.9%) markers is discussed. Spanning over 80% of the 2,470 cM estimated average length of the genome, the present map constitutes a useful research tool for molecular breeding and genomics projects in pineapple and other Bromeliaceae species.

Mots-clés Agrovoc : Ananas comosus, carte génétique, marqueur génétique, génomique, séquence nucléotidique

Mots-clés géographiques Agrovoc : Martinique, France

Mots-clés complémentaires : SSR

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Carlier Jorge Dias, Universidade do Algarve (PRT)
• Sousa Nelson Horta, Universidade do Algarve (PRT)
• Santo Tatiana Espirito, Universidade do Algarve (PRT)
• Coppens D'Eeckenbrugge Géo, CIRAD-BIOS-UMR CEFE (FRA) ORCID: 0000-0002-1970-0627
• Leitão José Manuel, Universidade do Algarve (PRT)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/564895/)

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