OsWRKY22, a monocot WRKY gene, plays a role in the resistance response to blast

Abbruscato Pamela, Nepusz Tamas, Mizzi Luca, Del Corvo Marcello, Morandini Piero, Fumasoni Irene, Michel Corinne, Paccanaro Alberto, Guiderdoni Emmanuel, Schaffrath Ulrich, Morel Jean-Benoit, Piffanelli Pietro, Faivre-Rampant Odile. 2012. OsWRKY22, a monocot WRKY gene, plays a role in the resistance response to blast. Molecular Plant Pathology, 13 (8) : 828-841.

Résumé : With the aim of identifying novel regulators of host and nonhost resistance to fungi in rice, we carried out a systematic mutant screen of mutagenized lines. Two mutant wrky22 knockout lines revealed clear-cut enhanced susceptibility to both virulent and avirulent Magnaporthe oryzae strains and altered cellular responses to nonhost Magnaporthe grisea and Blumeria graminis fungi. In addition, the analysis of the pathogen responses of 24 overexpressor OsWRKY22 lines revealed enhanced resistance phenotypes on infection with virulent M. oryzae strain, confirming that OsWRKY22 is involved in rice resistance to blast. Bioinformatic analyses determined that the OsWRKY22 gene belongs to a well-defined cluster of monocotspecific WRKYs. The co-regulatory analysis revealed no significant co-regulation of OsWRKY22 with a representative panel of OsWRKYs, supporting its unique role in a series of transcriptional responses. In contrast, inquiring a subset of biotic stress-related Affymetrix data, a large number of resistance and defencerelated genes were found to be putatively co-expressed with OsWRKY22. Taken together, all gathered experimental evidence places the monocot-specific OsWRKY22 gene at the convergence point of signal transduction circuits in response to both host and nonhost fungi encountering rice plants.

Mots-clés Agrovoc : Oryza sativa, Hordeum vulgare, Magnaporthe, Magnaporthe grisea, Blumeria graminis, gène, bioinformatique, mutant, résistance aux maladies

Mots-clés complémentaires : Magnaporthe oryzae

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Abbruscato Pamela, Parco Tecnologico Padano (ITA)
• Nepusz Tamas, University of London (GBR)
• Mizzi Luca, University of Milan (ITA)
• Del Corvo Marcello, Parco Tecnologico Padano (ITA)
• Morandini Piero, University of Milan (ITA)
• Fumasoni Irene, Parco Tecnologico Padano (ITA)
• Michel Corinne, INRA (FRA)
• Paccanaro Alberto, University of London (GBR)
• Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
• Schaffrath Ulrich, RWTHA (DEU)
• Morel Jean-Benoit, INRA (FRA)
• Piffanelli Pietro, Parco Tecnologico Padano (ITA)
• Faivre-Rampant Odile, INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/565328/)

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