SALT-RESPONSIVE ERF1 is a negative regulator of grain filling and gibberellin-mediated seedling establishment in rice

Schmidt Romy, Schippers Jos H.M., Mieulet Delphine, Watanabe Mutsumi, Hoefgen Rainer, Guiderdoni Emmanuel, Mueller-Roeber Bernd. 2014. SALT-RESPONSIVE ERF1 is a negative regulator of grain filling and gibberellin-mediated seedling establishment in rice. Molecular Plant, 7 (2) : 404-421.

Quartile : Outlier, Sujet : PLANT SCIENCES / Quartile : Q1, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Résumé : Grain quality is an important agricultural trait that is mainly determined by grain size and composition. Here, we characterize the role of the rice transcription factor (TF) SALT-RESPONSIVE ERF1 (SERF1) during grain development. Through genome-wide expression profiling and chromatin immunoprecipitation, we found that SERF1 directly regulates RICE PROLAMIN-BOX BINDING FACTOR (RPBF), a TF that functions as a positive regulator of grain filling. Loss of SERF1 enhances RPBF expression resulting in larger grains with increased starch content, while SERF1 overexpression represses RPBF resulting in smaller grains. Consistently, during grain filling, starch biosynthesis genes such as GRANULE-BOUND STARCH SYNTHASEI (GBSSI), STARCH SYNTHASEI (SSI), SSIIIa, and ADP-GLUCOSE PYROPHOSPHORYLASE LARGE SUBUNIT2 (AGPL2) are up-regulated in SERF1 knockout grains. Moreover, SERF1 is a direct upstream regulator of GBSSI. In addition, SERF1 negatively regulates germination by controlling RPBF expression, which mediates the gibberellic acid (GA)-induced expression of RICE AMYLASE1A (RAmy1A). Loss of SERF1 results in more rapid seedling establishment, while SERF1 overexpression has the opposite effect. Our study reveals that SERF1 represents a negative regulator of grain filling and seedling establishment by timing the expression of RPBF.

Mots-clés Agrovoc : Oryza sativa, riz, qualité, amidon, grain, développement biologique, transcription, génétique moléculaire

Mots-clés complémentaires : Amidon de riz

Classification Agris : F60 - Physiologie et biochimie végétale
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

• Schmidt Romy, University of Potsdam (DEU)
• Schippers Jos H.M., University of Potsdam (DEU)
• Mieulet Delphine, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-6220-0372
• Watanabe Mutsumi, Max Planck Institute of Molecular Plant Physiology (DEU)
• Hoefgen Rainer, Max Planck Institute of Molecular Plant Physiology (DEU)
• Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
• Mueller-Roeber Bernd, University of Potsdam (DEU)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/572920/)

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