Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme

Gallage Nethaji J., Hansen Esben H., Kannangara Rubini, Olsen Carl Erik, Motawia Mohammed Saddik, Jorgensen Kirsten, Holme Inger, Hebelstrup Kim, Grisoni Michel, Moller Birger Lindberg. 2014. Vanillin formation from ferulic acid in Vanilla planifolia is catalysed by a single enzyme. Nature Communications, 5 (4037), 14 p.

https://doi.org/10.1038/ncomms5037

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Quartile : Outlier, Sujet : MULTIDISCIPLINARY SCIENCES

Résumé : Vanillin is a popular and valuable flavour compound. It is the key constituent of the natural vanilla flavour obtained from cured vanilla pods. Here we show that a single hydratase/lyase type enzyme designated vanillin synthase (VpVAN) catalyses direct conversion of ferulic acid and its glucoside into vanillin and its glucoside, respectively. The enzyme shows high sequence similarity to cysteine proteinases and is specific to the substitution pattern at the aromatic ring and does not metabolize caffeic acid and p-coumaric acid as demonstrated by coupled transcription/translation assays. VpVAN localizes to the inner part of the vanilla pod and high transcript levels are found in single cells located a few cell layers from the inner epidermis. Transient expression of VpVAN in tobacco and stable expression in barley in combination with the action of endogenous alcohol dehydrogenases and UDP-glucosyltransferases result in vanillyl alcohol glucoside formation from endogenous ferulic acid. A gene encoding an enzyme showing 71% sequence identity to VpVAN was identified in another vanillin-producing plant species Glechoma hederacea and was also shown to be a vanillin synthase as demonstrated by transient expression in tobacco.

Mots-clés Agrovoc : Vanilla planifolia, vanilline, acide férulique, biosynthèse, activité enzymatique, ligase, Glechoma, Nicotiana

Mots-clés géographiques Agrovoc : La Réunion, France

Mots-clés complémentaires : Nicotiana benthamiana

Classification Agris : F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

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