Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.

Mérabet Chahinez, Martens M., Mahdhi Mosbah, Zakhia Frédéric, Sy Abdoulaye, Le Roux Christine, Domergue Odile, Coopman R., Bekki Abdelkader, Mars Messaoud, Willems Anne, De Lajudie Philippe. 2010. Multilocus sequence analysis of root nodule isolates from Lotus arabicus (Senegal), Lotus creticus, Argyrolobium uniflorum and Medicago sativa (Tunisia) and description of Ensifer numidicus sp. nov. and Ensifer garamanticus sp. nov.. International Journal of Systematic and Evolutionary Microbiology, 60 (3) : 664-674.

https://doi.org/10.1099/ijs.0.012088-0

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Quartile : Q3, Sujet : MICROBIOLOGY

Résumé : Nine isolates from Argyrolobium uniflorum, Lotus creticus, Medicago sativa (Tunisia) and Lotus arabicus (Senegal) were analysed by multilocus sequence analysis (MLSA) of five housekeeping genes (recA, atpD, glnA, gltA and thrC), the 16S rRNA gene and the nodulation gene nodA. Analysis of the individual and concatenated gene sequences demonstrated that the nine new strains constituted three stable, well-supported (bootstrap and gene sequence similarity values) monophyletic clusters, A, B and C, all belonging to the branch of the genus Ensifer, regardless of the phylogenetic reconstruction method used (maximum likelihood, maximum-parsimony, neighbour-joining). The three groups were further characterized by API 100 auxanographic tests, host specificity and nodA gene sequence analysis. On the basis of these data, clusters A and C are suggested as representing two novel species within the genus Ensifer, for which the names Ensifer numidicus sp. nov. (type strain ORS 1407T5LMG 24690T5CIP 109850T) and Ensifer garamanticus sp. nov. (type strain ORS 1400T5LMG 24692T5CIP 109916T) are proposed. The cluster B strains were assigned to Ensifer adhaerens genomovar A.

Mots-clés Agrovoc : Rhizobactérie, Rhizobiaceae, génie génétique, taxonomie, Lotus, Medicago sativa, séquence nucléotidique, système racinaire

Mots-clés géographiques Agrovoc : Tunisie, Sénégal

Mots-clés complémentaires : Souche (organisme), Ensifer garamanticus, Ensifer numidicus, Ensifer adhaerens, Argyrolobium uniflorum, Lotus creticus

Classification Agris : P34 - Biologie du sol
F60 - Physiologie et biochimie végétale
F61 - Physiologie végétale - Nutrition

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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