The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice

Conrad Liza J., Khanday Imtiyaz, Johnson Cameron, Guiderdoni Emmanuel, An Gynheung, Vijayraghavan Usha, Sundaresan Venkatesan. 2014. The polycomb group gene EMF2B is essential for maintenance of floral meristem determinacy in rice. Plant Journal, 80 (5) : 883-894.

Quartile : Outlier, Sujet : PLANT SCIENCES

Résumé : Polycomb Repressive Complex 2 (PRC2) represses the transcriptional activity of target genes through trimethylation of lysine 27 of histone H3. The functions of plant PRC2 have been chiefly described in Arabidopsis, but specific functions in other plant species, especially cereals, are still largely unknown. Here we characterize mutants in the rice EMF2B gene, an ortholog of the Arabidopsis EMBRYONIC FLOWER2 (EMF2) gene. Loss of EMF2B in rice results in complete sterility, and mutant flowers have severe floral organ defects and indeterminacy that resemble loss-of-function mutants in E-function floral organ specification genes. Transcriptome analysis identified the E-function genes OsMADS1, OsMADS6 and OsMADS34 as differentially expressed in the emf2b mutant compared with wild type. OsMADS1 and OsMADS6, known to be required for meristem determinacy in rice, have reduced expression in the emf2b mutant, whereas OsMADS34 which interacts genetically with OsMADS1 was ectopically expressed. Chromatin immunoprecipitation for H3K27me3 followed by quantitative (q)RT-PCR showed that all three genes are presumptive targets of PRC2 in the meristem. Therefore, in rice, and possibly other cereals, PRC2 appears to play a major role in floral meristem determinacy through modulation of the expression of E-function genes.

Mots-clés Agrovoc : Oryza sativa, méristème, inflorescence, physiologie de la reproduction, gène, Transcription génique, expression des gènes, Arabidopsis thaliana

Classification Agris : F30 - Génétique et amélioration des plantes
F63 - Physiologie végétale - Reproduction
F50 - Anatomie et morphologie des plantes

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

• Conrad Liza J., UCR (USA)
• Khanday Imtiyaz, IISC (IND)
• Johnson Cameron, UCR (USA)
• Guiderdoni Emmanuel, CIRAD-BIOS-UMR AGAP (FRA)
• An Gynheung, Kyung Hee University (KOR)
• Vijayraghavan Usha, IISC (IND)
• Sundaresan Venkatesan, UCR (USA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/574720/)

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