Highly polymorphic markers reveal the establishment of an invasive lineage of the citrus bacterial pathogen Xanthomonas citri pv. citri in its area of origin

Vernière Christian, Bui Thi Ngoc Lan, Jarne Philippe, Ravigné Virginie, Guérin Fabien, Gagnevin Lionel, Le Mai Nhat, Chau Nguyen M., Pruvost Olivier. 2014. Highly polymorphic markers reveal the establishment of an invasive lineage of the citrus bacterial pathogen Xanthomonas citri pv. citri in its area of origin. Environmental Microbiology, 16 (7) : 2226-2237.

https://doi.org/10.1111/1462-2920.12369

Article de revue ; Article de revue à facteur d'impact

Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
document_574888.pdf
Télécharger (442kB)

Quartile : Q1, Sujet : MICROBIOLOGY

Résumé : Investigating the population biology of plant pathogens in their native areas is essential to understand the factors that shape their population structure and favour their spread. Monomorphic pathogens dispatch extremely low genetic diversity in invaded areas, and native areas constitute a major reservoir for future emerging strains. One of these, the gammaproteobacterium Xanthomonas citri pv. citri, causes Asiatic canker and is a considerable threat to citrus worldwide. We studied its population genetic structure by genotyping 555 strains from 12 Vietnam provinces at 14 tandem repeat loci and insertion sequences. Discriminant analysis of principal components identified six clusters. Five of them were composed of endemic strains distributed heterogeneously across sampled provinces. A sixth cluster, VN6, displayed a much lower diversity and a clonal expansion structure, suggesting recent epidemic spread. No differences in aggressiveness on citrus or resistance to bactericides were detected between VN6 and other strains. VN6 likely represents a case of bioinvasion following introduction in a native area likely through contaminated plant propagative material. Highly polymorphic markers are useful for revealing migration patterns of recently introduced populations of a monomorphic bacterial plant pathogen.

Mots-clés Agrovoc : Xanthomonas campestris citri, marqueur génétique, polymorphisme génétique, pathotype, évolution, épidémiologie, développement biologique, variation génétique, provenance, distribution géographique, génétique des populations, pouvoir pathogène, Rutaceae, Citrus