Agritrop
Home

Free exopolysaccharide from Mycoplasma mycoides subsp. mycoides possesses anti-inflammatory properties

Totté Philippe, Puech Carinne, Rodrigues Valérie, Bertin Clothilde, Manso-Silvan Lucia, Thiaucourt François. 2015. Free exopolysaccharide from Mycoplasma mycoides subsp. mycoides possesses anti-inflammatory properties. Veterinary Research, 46 (122), 9 p.

Journal article ; Article de revue à facteur d'impact Revue en libre accès total
[img]
Preview
Published version - Anglais
Use under authorization by the author or CIRAD.
577940.pdf

Télécharger (1MB) | Preview

Quartile : Q1, Sujet : VETERINARY SCIENCES

Abstract : In this study we explored the immunomodulatory properties of highly purified free galactan, the soluble exopolysaccharide secreted by Mycoplasma mycoides subsp. mycoides (Mmm). Galactan was shown to bind to TLR2 but not TLR4 using HEK293 reporter cells and to induce the production of the anti-inflammatory cytokine IL-10 in bovine macrophages, whereas low IL-12p40 and no TNF-α, both pro-inflammatory cytokines, were induced in these cells. In addition, pre-treatment of macrophages with galactan substantially reduced lipopolysaccharide (LPS)-induced production of pro-inflammatory cytokines TNF- and IL-12p40 while increasing LPS-induced secretion of immunosuppressive IL-10. Also, galactan did not activate naïve lymphocytes and induced only low production of the Th1 cytokine IFN-γ in Mmm-experienced lymphocytes. Finally, galactan triggered weak recall proliferation of CD4+ T lymphocytes from contagious bovine pleuropneumonia-infected animals despite having a positive effect on the expression of co-stimulatory molecules on macrophages. All together, these results suggest that galactan possesses anti-inflammatory properties and potentially provides Mmm with a mechanism to evade host innate and adaptive cell-mediated immune responses. (Résumé d'auteur)

Mots-clés Agrovoc : Mycoplasma mycoides, Polyholoside, Anti-inflammatoire, Galactane, Cytokinine, Macrophage, Lymphocyte, Bovin

Mots-clés géographiques Agrovoc : Languedoc-Roussillon

Classification Agris : L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Totté Philippe, CIRAD-BIOS-UMR CMAEE (FRA)
  • Puech Carinne, INRA (FRA)
  • Rodrigues Valérie, CIRAD-BIOS-UMR CMAEE (FRA)
  • Bertin Clothilde, INRA (FRA)
  • Manso-Silvan Lucia, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0002-5539-3804
  • Thiaucourt François, CIRAD-BIOS-UMR CMAEE (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/577940/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2020-10-30 ]