Genome-wide association mapping for root traits in a panel of rice accessions from Vietnam

Phung Nhung Thi Phuong, Mai Chung Duc, Hoang Giang Thi, Truong Hue Thi Minh, Lavarenne Jeremy, Gonin Mathieu, Nguyen Khanh L., Ha Thuy Thi, Do Vinh Nang, Gantet Pascal, Courtois Brigitte. 2016. Genome-wide association mapping for root traits in a panel of rice accessions from Vietnam. BMC Plant Biology, 16 (1), 19 p.

https://doi.org/10.1186/s12870-016-0747-y

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Url - autres données associées : http://tropgenedb.cirad.fr/tropgene/JSP/interface.jsp?module=RICE

Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Background. Despite recent sequencing efforts, local genetic resources remain underexploited, even though they carry alleles that can bring agronomic benefits. Taking advantage of the recent genotyping with 22,000 single-nucleotide polymorphism markers of a core collection of 180 Vietnamese rice varieties originating from provinces from North to South Vietnam and from different agrosystems characterized by contrasted water regimes, we have performed a genome-wide association study for different root parameters. Roots contribute to water stress avoidance and are a still underexploited target for breeding purpose due to the difficulty to observe them. Results The panel of 180 rice varieties was phenotyped under greenhouse conditions for several root traits in an experimental design with 3 replicates. The phenotyping system consisted of long plastic bags that were filled with sand and supplemented with fertilizer. Root length, root mass in different layers, root thickness, and the number of crown roots, as well as several derived root parameters and shoot traits, were recorded. The results were submitted to association mapping using a mixed model involving structure and kinship to enable the identification of significant associations. The analyses were conducted successively on the whole panel and on its indica (115 accessions) and japonica (64 accessions) subcomponents. The two associations with the highest significance were for root thickness on chromosome 2 and for crown root number on chromosome 11. No common associations were detected between the indica and japonica subpanels, probably because of the polymorphism repartition between the subspecies. Based on orthology with Arabidopsis, the possible candidate genes underlying the quantitative trait loci are reviewed. Conclusions Some of the major quantitative trait loci we detected through this genome-wide association study contain promising candidate genes encoding regulatory elements of known key regulators of root formation and development.

Mots-clés Agrovoc : Oryza sativa, génome, génotype, carte génétique, développement biologique, système racinaire, racine, variété, locus des caractères quantitatifs, marqueur génétique

Mots-clés géographiques Agrovoc : Viet Nam

Mots-clés complémentaires : Séquencage, QTL, Oryza sativa indica, Oryza sativa japonica

Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

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