First detection of a Mesonivirus in Culex pipens in five countries around the Mediterranean basin

Gil Patricia, Rakotoarivony Ignace, Loire Etienne, Marie Albane, Francés Benoit, L'Ambert Grégory, Busquets-Martí Núria, Birnberg Lotty, Talavera Sandra, Carlos Aranda, Bessat Mohamed, Otify Yehia, Mansini Giuseppe, Goffredo Maria, Ayari Emna, Mejri Selma, Muth Erika, Cheval Justine, De Visser Louise, Lamany Arnaud, Cabannes Eric, Hebert Charles, Eloit Marc, Gutierrez Serafin. 2017. First detection of a Mesonivirus in Culex pipens in five countries around the Mediterranean basin. In : Abstract book of the 11th Epizone Annual Meeting welcome in Paris. ANSES. Paris : ANSES, Résumé, 27. Annual Meeting EPIZONE: Crossing barriers. 11, Paris, France, 19 Septembre 2017/21 Septembre 2017.

Résumé : Culex pipiens is a mosquito vector of West Nile virus (WNV), the causing agent of West Nile fever. Epizooties of WNV have taken place in Europe, mainly in France, Italy and Spain, and in some instances, human cases have been diagnosed. However, beyond WNV, little is known on the viruses associated to Culex pipiens, above all on viruses non-pathogenic for humans or livestock. Like Wolbachia bacteria, such viruses could have a role in WNV epidemiology either limiting mosquito populations or modulating the chances of WNV infection of C. pipiens. Through metagenomics approach, we characterized the virome of C. pipiens in five countries around the Mediterranean basin Our results show for the first time conserved patterns in diversity and structure within the virome of C. pipiens with a large abundance of the Mesoniviridae, a relatively new family of mosquito-specific viruses in the Nidovirales order. To date, the few data available on Mesoniviridae show that they probably have a worldwide distribution and they can infect different species of mosquitoes. The sequences obtained allowed to assemble several full genomes of Mesoniviridae. Interestingly, phylogenetic analysis showed a highly similarity with strains of Nam-Dinh virus, a virus recently identified in Côte d'Ivoire (West Africa) and Vietnam but which had not yet been observed in Europe. In view of the importance of the Mesoniviridae dispersion throughout the world, we determined the relative abundance of this family in natural population of Culex pipiens in Camargue. (Texte integral)

Mots-clés libres : Culex pipiens, West Nile virus, Virome, Metagenomics, Mesoniviridae, Insect specific virus

Classification Agris : L73 - Maladies des animaux
L72 - Organismes nuisibles des animaux
000 - Autres thèmes

Auteurs et affiliations

• Gil Patricia, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0001-7984-7340
• Rakotoarivony Ignace, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0009-0002-3480-6734
• Loire Etienne, CIRAD-BIOS-UMR ASTRE (FRA)
• Marie Albane, EID (FRA)
• Francés Benoit, EID (FRA)
• L'Ambert Grégory, EID (FRA)
• Busquets-Martí Núria, CReSA (ESP)
• Birnberg Lotty, CReSA (ESP)
• Talavera Sandra, CReSA (ESP)
• Carlos Aranda, CReSA (ESP)
• Bessat Mohamed, Alexandria University (EGY)
• Otify Yehia, Alexandria University (EGY)
• Mansini Giuseppe, IZSA&M (ITA)
• Goffredo Maria, IZSA&M (ITA)
• Ayari Emna, Institut Pasteur de Tunis (TUN)
• Mejri Selma, Institut Pasteur de Tunis (TUN)
• Muth Erika, PathoQuest (FRA)
• Cheval Justine, PathoQuest (FRA)
• De Visser Louise, PathoQuest (FRA)
• Lamany Arnaud, PathoQuest (FRA)
• Cabannes Eric, PathoQuest (FRA)
• Hebert Charles, PathoQuest (FRA)
• Eloit Marc, Institut Pasteur (FRA)
• Gutierrez Serafin, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0002-5277-7239

Source : Cirad-Agritrop (https://agritrop.cirad.fr/585517/)

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