Differentiation and quantification of the ochratoxin A producers Aspergillus ochraceus and Aspergillus westerdijkiae using PCR-DGGE

Durand Noel, Fontana Angélique, Meile Jean-Christophe, Suárez-Quiroz Mirna, Schorr-Galindo Sabine, Montet Didier. 2019. Differentiation and quantification of the ochratoxin A producers Aspergillus ochraceus and Aspergillus westerdijkiae using PCR-DGGE. Journal of Basic Microbiology, 59 (2) : 158-165.

Quartile : Q3, Sujet : MICROBIOLOGY

Résumé : Ochratoxin A (OTA) is a nephrotoxic, teratogenic, immunotoxic, and carcinogenic mycotoxin which is produced in tropical zones mainly by Aspergillus carbonarius, A. niger, A. ochraceus, and A. westerdijkiae. A. ochraceus and A. westerdijkiae species are phenotypically and genomically very close but A. westerdijkiae produce OTA at a very higher level than A. ochraceus. These species have been differentiated recently. The DNA primer pairs which were drawn so far are not specific and a genomic region of the same size is amplified for both species or they are too specific, and in this case, the DNA of a single species is amplified. To help preventing OTA contamination of foodstuffs, the PCR‐DGGE (Denaturing Gradient Gel Electrophoresis) method was used to discriminate between A. ochraceus and A. westerdijkiae DNA fragments of the same size but with different sequences and thus faster access to a diagnosis of the toxigenic potential of the fungal microflora. The proposed methodology was able to differentiate A. westerdijkiae from A. ochraceus with only one primer pairs in a single run. A calibration based on initial DNA content was obtained from image analysis of the DGGE gels and a method of quantification of the two strains was proposed.

Mots-clés Agrovoc : ochratoxine, Aspergillus ochraceus, électrophorèse sur gel

Mots-clés complémentaires : Aspergillus westerdijkiae

Mots-clés libres : Aspergillus westerdijkiae, Image analysis, Ochratoxin A, PCR-DGGE

Classification Agris : Q03 - Contamination et toxicologie alimentaires
S50 - Santé humaine

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

Auteurs et affiliations

• Durand Noel, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0003-1627-6848
• Fontana Angélique, UM2 (FRA) - auteur correspondant
• Meile Jean-Christophe, CIRAD-PERSYST-UMR Qualisud (REU) ORCID: 0000-0002-6747-2104
• Suárez-Quiroz Mirna, Instituto Tecnologico de Veracruz (MEX)
• Schorr-Galindo Sabine, UM2 (FRA)
• Montet Didier, CIRAD-PERSYST-UMR Qualisud (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/589202/)

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