Inhibition of Phytophthora species, agents of cocoa black pod disease, by secondary metabolites of Trichoderma species

Pakora Gilles-Alex, Mpika Joseph, Koné Daouda, Ducamp Michel, Kébé Ismael S., Nay Bastien, Buisson Didier. 2018. Inhibition of Phytophthora species, agents of cocoa black pod disease, by secondary metabolites of Trichoderma species. Environmental Science and Pollution Research, 25 (30) : 29901-29909.

https://doi.org/10.1007/s11356-017-0283-9

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Quartile : Q2, Sujet : ENVIRONMENTAL SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Cocoa production is affected by the black pod disease caused by several Phytophthora species that bring, about each year, an estimated loss of 44% of world production. Chemical control remains expensive and poses an enormous risk of poisoning for the users and the environment. Biocontrol by using antagonistic microorganisms has become an alternative to the integrated control strategy against this disease. Trichoderma viride T7, T. harzanium T40, and T. asperellum T54, which showed in vivo and in vitro antagonistic activity against P. palmivora, were cultured and mycelia extracted. Inhibition activity of crude extracts was determined, and then organic compounds were isolated and characterized. The in vitro effect of each compound on the conidia germination and mycelia growth of four P. palmivora, two P. megakaria, and one P. capsici was evaluated. T. viride that displayed best activities produced two active metabolites, viridin and gliovirin, against P. palmivora and P. megakaria strains. However, no activity against P. capsici was observed. Besides being active separately, these two compounds have a synergistic effect for both inhibitions, mycelia growth and conidia germination. These results provide the basis for the development of a low-impact pesticide based on a mixture of viridin and gliovirine.

Mots-clés Agrovoc : Theobroma cacao, Phytophthora capsici, Phytophthora palmivora, Phytophthora, Trichoderma viride, Trichoderma harzianum, métabolite secondaire, maladie des plantes

Mots-clés complémentaires : Phytophthora megakarya, Trichoderma asperellum

Mots-clés libres : Cacao, Phytophthora, Trichoderma virens, Viridin, Gliovirin, Synergistic effect

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

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