Phenotypic characterization of indigenous Saccharomyces cerevisiae strains associated with sorghum beer and palm wines

Tra Bi Charles Y., Kouakou-Kouamé Clémentine Amenan, N'Guessan Florent Kouadio, Djè Marcellin, Montet Didier. 2021. Phenotypic characterization of indigenous Saccharomyces cerevisiae strains associated with sorghum beer and palm wines. World Journal of Microbiology and Biotechnology, 37 (2):24, 12 p.

Quartile : Q2, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Résumé : In order to phenotypically characterized Saccharomyces cerevisiae strains isolated from sorghum beer and palm wines for a possible selection of a starter culture, 30 strains were tested for killer activity, temperature resistance, ethanol tolerance, carbohydrate fermentation, enzyme profile and sorghum wort fermentation. Of the tested strains, three showed a killer profile, while four showed a neutral profile and 23 were found to be sensitive to K2 toxin. Temperatures of 40 °C and 44 °C allowed to distinguish strains into four thermal groups with only three strains may grow at 44 °C. Almost tested strains were tolerant to 5% ethanol with viability rates up to 73%. But at 10% and 15% ethanol, respectively 18 and 7 strains were tolerant. Carbohydrate fermentation revealed 13 fermentation profiles, including one typical and 12 atypical profiles. The typical profile strains (16.13% of the strains) fermented glucose, galactose, fructose, sucrose, maltose, trehalose and raffinose. Most of the strains secreted lipases (mainly esterase and esterase-lipase), proteases (mainly valine and cysteine arylamidase, chrymotrypsin) and phosphatases (mainly acid phosphatase and naphthol phosphohydrolase). On contrary, only five strains isolated from sorghum beer exhibited glucosidase activity, mainly α-glucosidase. The analyse of fermented sorghum wort revealed that fermentative performance is strain dependent. Furthermore, the Hierarchical Cluster Analysis showed that the strains were separated in three distinct clusters with the strains from sorghum beer clustered separately.

Mots-clés Agrovoc : Saccharomyces cerevisiae, boisson alcoolisée, bière, vin, phénotype, fermentation, culture starter

Mots-clés complémentaires : Vin de palme, Bière de mil

Mots-clés libres : Fermentation, Palm wines, Phenotypic diversity, Saccharomyces cerevisiae strains, Starter culture, Sorghum beer

Classification Agris : Q01 - Sciences et technologies alimentaires - Considérations générales
Q04 - Composition des produits alimentaires

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

Auteurs et affiliations

• Tra Bi Charles Y., UNA [Université Nangui Abrogoua] (CIV)
• Kouakou-Kouamé Clémentine Amenan, UNA [Université Nangui Abrogoua] (CIV)
• N'Guessan Florent Kouadio, UNA [Université Nangui Abrogoua] (CIV) - auteur correspondant
• Djè Marcellin, UNA [Université Nangui Abrogoua] (CIV)
• Montet Didier, CIRAD-PERSYST-UMR Qualisud (FRA)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/597384/)

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