Microwave-assisted one-pot lipid extraction and glycolipid production from oleaginous yeast Saitozyma podzolica in sugar alcohol-based media

Delavault André, Ochs Katarina, Gorte Olga, Syldatk Christoph, Durand Erwann, Ochsenreither Katrin. 2021. Microwave-assisted one-pot lipid extraction and glycolipid production from oleaginous yeast Saitozyma podzolica in sugar alcohol-based media. Molecules, 26:470, 16 p.

Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY / Quartile : Q2, Sujet : CHEMISTRY, MULTIDISCIPLINARY

Résumé : Glycolipids are non-ionic surfactants occurring in numerous products of daily life. Due to their surface-activity, emulsifying properties, and foaming abilities, they can be applied in food, cosmetics, and pharmaceuticals. Enzymatic synthesis of glycolipids based on carbohydrates and free fatty acids or esters is often catalyzed using certain acyltransferases in reaction media of low water activity, e.g., organic solvents or notably Deep Eutectic Systems (DESs). Existing reports describing integrated processes for glycolipid production from renewables use many reaction steps, therefore this study aims at simplifying the procedure. By using microwave dielectric heating, DESs preparation was first accelerated considerably. A comparative study revealed a preparation time on average 16-fold faster than the conventional heating method in an incubator. Furthermore, lipids from robust oleaginous yeast biomass were successfully extracted up to 70% without using the pre-treatment method for cell disruption, limiting logically the energy input necessary for such process. Acidified DESs consisting of either xylitol or sorbitol and choline chloride mediated the one-pot process, allowing subsequent conversion of the lipids into mono-acylated palmitate, oleate, linoleate, and stearate sugar alcohol esters. Thus, we show strong evidence that addition of immobilized Candida antarctica Lipase B (Novozym 435®), in acidified DES mixture, enables a simplified and fast glycolipid synthesis using directly oleaginous yeast biomass.

Mots-clés Agrovoc : glycolipide, micro-ondes, sucre alcool, lipase

Mots-clés complémentaires : Saitozyma podzolica, solvant eutectique profond, levure oléagineuse, Candida antarctica

Mots-clés libres : Glycolipid, Single cell oil, Lipase, Microwave, One-pot process, Deep eutectic solvents

Classification Agris : F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : CTS 7 (2019-) - Hors champs stratégiques

Auteurs et affiliations

• Delavault André, Karlsruhe Institute of Technology (DEU) - auteur correspondant
• Ochs Katarina, Karlsruhe Institute of Technology (DEU)
• Gorte Olga, Karlsruhe Institute of Technology (DEU)
• Syldatk Christoph, Karlsruhe Institute of Technology (DEU)
• Durand Erwann, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0002-0306-8081
• Ochsenreither Katrin, Karlsruhe Institute of Technology (DEU)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/597611/)

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