Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing

Belser Caroline, Baurens Franc-Christophe, Noël Benjamin, Martin Guillaume, Cruaud Corinne, Istace Benjamin, Yahiaoui Nabila, Labadie Karine, Hribova Eva, Dolezel Jaroslav, Lemainque Arnaud, Wincker Patrick, D'Hont Angélique, Aury Jean-Marc. 2021. Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing. Communications Biology, 4:1047, 12 p.

https://doi.org/10.1038/s42003-021-02559-3

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
Belser et al 2021_Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing.pdf
Télécharger (2MB) | Prévisualisation

Url - autres données associées : https://doi.org/10.5281/zenodo.5120019

Quartile : Q1, Sujet : BIOLOGY

Résumé : Long-read technologies hold the promise to obtain more complete genome assemblies and to make them easier. Coupled with long-range technologies, they can reveal the architecture of complex regions, like centromeres or rDNA clusters. These technologies also make it possible to know the complete organization of chromosomes, which remained complicated before even when using genetic maps. However, generating a gapless and telomere-to-telomere assembly is still not trivial, and requires a combination of several technologies and the choice of suitable software. Here, we report a chromosome-scale assembly of a banana genome (Musa acuminata) generated using Oxford Nanopore long-reads. We generated a genome coverage of 177X from a single PromethION flowcell with near 17X with reads longer than 75 kbp. From the 11 chromosomes, 5 were entirely reconstructed in a single contig from telomere to telomere, revealing for the first time the content of complex regions like centromeres or clusters of paralogous genes.

Mots-clés Agrovoc : phytogénétique, génome, télomères, biotechnologie végétale, génomique, Musa acuminata

Mots-clés libres : Genome informatics, Genomics, Plant biotechnology

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Auteurs et affiliations