An atypical class of non-coding small RNAs is produced in rice leaves upon bacterial infection

Reshetnyak Ganna, Jacobs Jonathan M., Auguy Florence, Sciallano Coline, Claude Lisa, Medina Clemence, Pérez-Quintero Alvaro L., Comte Aurore, Thomas Emilie, Bogdanove Adam J., Koebnik Ralf, Szurek Boris, Diévart Anne, Brugidou Christophe, Lacombe Séverine, Cunnac Sébastien. 2021. An atypical class of non-coding small RNAs is produced in rice leaves upon bacterial infection. Scientific Reports, 11 (1):24141, 20 p.

https://doi.org/10.1038/s41598-021-03391-9

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Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/bioproject/PRJNA427491 / Url - jeu de données - Entrepôt autre : https://mpss.danforthcenter.org/web/php/pages/library_info.php?SITE=rice_sRNA&showAll=true / Url - jeu de données - Entrepôt autre : https://dataverse.ird.fr/dataset.xhtml?persistentId=doi:10.23708/RXIXM3

Quartile : Q2, Sujet : MULTIDISCIPLINARY SCIENCES

Résumé : Non-coding small RNAs (sRNA) act as mediators of gene silencing and regulate plant growth, development and stress responses. Early insights into plant sRNAs established a role in antiviral defense and they are now extensively studied across plant–microbe interactions. Here, sRNA sequencing discovered a class of sRNA in rice (Oryza sativa) specifically associated with foliar diseases caused by Xanthomonas oryzae bacteria. Xanthomonas-induced small RNAs (xisRNAs) loci were distinctively upregulated in response to diverse virulent strains at an early stage of infection producing a single duplex of 20–22 nt sRNAs. xisRNAs production was dependent on the Type III secretion system, a major bacterial virulence factor for host colonization. xisRNA loci overlap with annotated transcripts sequences, with about half of them encoding protein kinase domain proteins. A number of the corresponding rice cis-genes have documented functions in immune signaling and xisRNA loci predominantly coincide with the coding sequence of a conserved kinase motif. xisRNAs exhibit features of small interfering RNAs and their biosynthesis depend on canonical components OsDCL1 and OsHEN1. xisRNA induction possibly mediates post-transcriptional gene silencing but they do not broadly suppress cis-genes expression on the basis of mRNA-seq data. Overall, our results identify a group of unusual sRNAs with a potential role in plant–microbe interactions.

Mots-clés Agrovoc : Oryza sativa, Xanthomonas oryzae, locus, gène, biosynthèse

Mots-clés libres : Plant immunity, Plant molecular biology, Small RNAs

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

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