CRISPR/Cas9-Targeted knockout of rice susceptibility genes OsDjA2 and OsERF104 reveals alternative sources of resistance to Pyricularia oryzae

Tavora Fabiano T.P.K., Meunier Anne Cecile, Vernet Aurore, Portefaix Murielle, Milazzo Joëlle, Adreit Henri, Tharreau Didier, Franco Octávio L., Mehta Angela. 2022. CRISPR/Cas9-Targeted knockout of rice susceptibility genes OsDjA2 and OsERF104 reveals alternative sources of resistance to Pyricularia oryzae. Rice Science, 29 (6) : 535-544.

http://www.ricescience.org/EN/abstract/abstract10026.shtml

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Résumé : Rice blast, caused by Pyricularia oryzae, is one of the most destructive diseases in agriculture leading to severe impacts on rice crop harvests worldwide. In a previous study, we showed that rice genes OsDjA2 and OsERF104, encoding a chaperone protein and an APETELA2/ethylene-responsive factor, respectively, were strongly induced in a compatible interaction with blast fungus, and also had their function in plant susceptibility validated through gene silencing. Here, we report the CRISPR/Cas9 knockout of OsDjA2 and OsERF104 genes resulting in considerable improvement of blast resistance. A total of 15 OsDjA2 (62.50%) and 17 OsERF104 (70.83%) T0 transformed lines were identified from 24 regenerated plants for each target and used in downstream experiments. Phenotyping of homozygous T1 mutant lines revealed not only a significant decrease in the number of blast lesions but also a reduction in the percentage of diseased leaf area, compared with the wild-type infected control plants. Our results supported CRISPR/Cas9-mediated target mutation in rice susceptibility genes as a potential and alternative breeding strategy for building resistance to blast disease.

Mots-clés Agrovoc : Pyricularia oryzae, pyriculariose du riz, Magnaporthe, Oryza, résistance aux maladies, immunité, gène, relation hôte pathogène, CRISPR

Mots-clés complémentaires : expérimentation sous serre

Mots-clés libres : Gene editing, Plant-pathogen interaction, Magnaporthe pathosystem, Plant immunity, Blast resistance, S-gene, Rice

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

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