Optimization of selective hydrolysis of cruciferins for production of potent mineral chelating peptides and napins purification to valorize total rapeseed meal proteins

Kaugarenia Nastassia, Beaubier Sophie, Durand Erwann, Aymes Arnaud, Villeneuve Pierre, Lesage François, Kapel Romain. 2022. Optimization of selective hydrolysis of cruciferins for production of potent mineral chelating peptides and napins purification to valorize total rapeseed meal proteins. Foods, 11 (17), n.spéc. High-Value Products from Food Wastes:2618, 16 p.

https://doi.org/10.3390/foods11172618

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
Beaubier Food 2022.pdf
Télécharger (1MB) | Prévisualisation

Résumé : Preventing oxidation and microbial spoilage are both major concerns in food industries. In this context, this study aimed to valorize the total rapeseed meal proteins with controlled enzymatic proteolysis to generate potent mineral-chelating peptides from cruciferins while keeping intact the antimicrobial napins. Implementation of proteolysis of total rapeseed protein isolate with the Prolyve® enzyme highlighted an interesting selective hydrolysis of the cruciferins. Hence, the mechanism of this particular hydrolysis was investigated through a Design of Experiments method to obtain a model for the prediction of kinetics (cruciferin degradation and napin purity) according to the operating conditions applied. Then, multicriteria optimization was implemented to maximize the napin purity and yield while minimizing both enzymatic cost and reaction time. Antioxidant assays of the peptide fraction obtained under the optimal conditions proved the high metal-chelating activity preservation (EC50 = 247 ± 27 µg) for more than three times faster production. This fraction might counteract lipid oxidation or serve as preventing agents for micronutrient deficiencies, and the resulting purified napins may have applications in food safety against microbial contamination. These results can greatly help the development of rapeseed meal applications in food industries.

Mots-clés libres : Antioxidant, Peptides, Colza

Classification Agris : Q70 - Traitement des déchets agricoles
Q02 - Traitement et conservation des produits alimentaires
Q52 - Traitement et conservation des aliments pour animaux

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

Auteurs et affiliations