Characterization of RTB starch grain size and shape through imaging. High-throughput phenotyping protocols (HTPP), WP3

Cornet Denis, Desfontaines Lucienne, Meghar Karima, Davrieux Fabrice. 2021. Characterization of RTB starch grain size and shape through imaging. High-throughput phenotyping protocols (HTPP), WP3. Montpellier : RTBfoods Project-CIRAD, 14 p.

https://doi.org/10.18167/agritrop/00670

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Résumé : Granules from different starch sources vary in composition, size and shape. The size distribution of starch grains and their shape contribute to some functional properties (e.g. viscosity, water retention capacity, starch gelatinization) but also certain mechanical properties. Conventional physicochemical measurements of starch properties are often costly and difficult to access. The work presented here aims to contribute to the evaluation of the quality of yam tubers through the development of an automated method for measuring the size and shape of starch grains in RTB products. This protocol is based on the detection of starch grains by iodine staining of starch from RTB flour. Once stained, a drop of the solution is observed under the microscope and an image is taken. A morphological segmentation based on the watershed algorithm is then applied to images in order to detect every starch grain. Finally, the grain starch size and shape are calculated based on appropriate scale and indicators.

Mots-clés libres : Food quality, Starch granule, Computer vision, Granule size determination, Morphology

Agences de financement hors UE : Bill and Melinda Gates Foundation, Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Centro Internacional de Agricultura Tropical, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, James Hutton Institute

Projets sur financement : (FRA) Breeding RTB Products for End User Preferences

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