Identification of a major QTL-Controlling resistance to the subtropical race 4 of Fusarium oxysporum f. sp. cubense in Musa acuminata ssp. malaccensis

Chen Andrew, Sun Jiaman, Martin Guillaume, Gray Lesley-Ann, Hribova Eva, Christelová Pavla, Yahiaoui Nabila, Rounsley Steve, Lyons Rebecca, Batley Jacqueline, Chen Ning, Hamill Sharon, Rai Subash K., Coin Lachlan, Uwimana Brigitte, D'Hont Angélique, Dolezel Jaroslav, Edwards David P., Swennen Rony, Aitken Elizabeth A. B.. 2023. Identification of a major QTL-Controlling resistance to the subtropical race 4 of Fusarium oxysporum f. sp. cubense in Musa acuminata ssp. malaccensis. Pathogens, 12 (2), n.spéc. Current Research on Fusarium:289, 13 p.

Résumé : Vascular wilt caused by the ascomycete fungal pathogen Fusarium oxysporum f. sp. cubense (Foc) is a major constraint of banana production around the world. The virulent race, namely Tropical Race 4, can infect all Cavendish-type banana plants and is now widespread across the globe, causing devastating losses to global banana production. In this study, we characterized Foc Subtropical Race 4 (STR4) resistance in a wild banana relative which, through estimated genome size and ancestry analysis, was confirmed to be Musa acuminata ssp. malaccensis. Using a self-derived F2 population segregating for STR4 resistance, quantitative trait loci sequencing (QTL-seq) was performed on bulks consisting of resistant and susceptible individuals. Changes in SNP index between the bulks revealed a major QTL located on the distal end of the long arm of chromosome 3. Multiple resistance genes are present in this region. Identification of chromosome regions conferring resistance to Foc can facilitate marker assisted selection in breeding programs and paves the way towards identifying genes underpinning resistance.

Mots-clés Agrovoc : Musa acuminata, Musa, génome, locus des caractères quantitatifs, Fusarium oxysporum, Musa (bananes), résistance génétique, collection de matériel génétique

Mots-clés libres : Banana, Fusarium wilt, Host resistance, Quantitative trait locus, Bulk segregant analysis, Fusarium oxysporum f. sp. cubense, Subtropical Race 4, QTL-seq

Classification Agris : H20 - Maladies des plantes
H01 - Protection des végétaux - Considérations générales

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Bill and Melinda Gates Foundation, Hort Innovation, Scientific Research and Technology Development Program of Guangxi, Natural Science Foundation of Guangxi Province

Auteurs et affiliations

• Chen Andrew, University of Queensland (AUS) - auteur correspondant
• Sun Jiaman, University of Queensland (AUS)
• Martin Guillaume, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-1801-7500
• Gray Lesley-Ann, Victorian Comprehensive Cancer Centre (AUS)
• Hribova Eva, Institute of Experimental Botany (CZE)
• Christelová Pavla, Institute of Experimental Botany (CZE)
• Yahiaoui Nabila, CIRAD-BIOS-UMR AGAP (FRA)
• Rounsley Steve, Inari Agriculture (USA)
• Lyons Rebecca, University of Queensland (AUS)
• Batley Jacqueline, University of Western Australia (AUS)
• Chen Ning, University of Queensland (AUS)
• Hamill Sharon, Maroochy Research Facility (AUS)
• Rai Subash K., University of Queensland (AUS)
• Coin Lachlan, University of Melbourne (AUS)
• Uwimana Brigitte, IITA (UGA)
• D'Hont Angélique, CIRAD-BIOS-UMR AGAP (FRA)
• Dolezel Jaroslav, Institute of Experimental Botany (CZE)
• Edwards David P., University of Western Australia (AUS)
• Swennen Rony, KUL (BEL)
• Aitken Elizabeth A. B., University of Queensland (AUS)

Source : Cirad-Agritrop (https://agritrop.cirad.fr/603818/)

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