Chelating peptides from rapeseed meal protein hydrolysates: identification and evaluation of their capacity to inhibit lipid oxidation

Beaubier Sophie, Durand Erwann, Lenclume Charles, Fine Frédéric, Aymes Arnaud, Framboisier Xavier, Kapel Romain, Villeneuve Pierre. 2023. Chelating peptides from rapeseed meal protein hydrolysates: identification and evaluation of their capacity to inhibit lipid oxidation. Food Chemistry, 422:136187, 9 p.

Résumé : An optimized proteolysis process was applied to rapeseed meal proteins (RP) and the hydrolysate was separated by membrane filtration allowing the production of highly metal-chelating peptides in the permeate. In order to identify the chemical structure of the most active obtained metal-chelating peptides, immobilized metal affinity chromatography (IMAC) was applied. The RP-IMAC peptide fraction was mainly composed of small peptides from 2 to 20 amino acids. Using the Ferrozine assay, RP-IMAC peptides showed a significant chelating efficiency higher than sodium citrate and close to that of EDTA. The peptide sequences were identified by UHPLC-MS and several possible iron binding sites were found. β-carotene oxidation assay and lipid oxidation in bulk oils or emulsion were carried out to evaluate the potential of such peptides as efficient antioxidants to protect lipids from oxidation. While chelating peptides showed a limited efficiency in bulk oil, they performed more efficiently in emulsion.

Mots-clés Agrovoc : hydrolysat de protéines, tourteau de colza, oxydation, chélateur

Mots-clés libres : Peptides, Rapeseed, Lipids, Oxidation, Antioxidant

Classification Agris : Q02 - Traitement et conservation des produits alimentaires
Q52 - Traitement et conservation des aliments pour animaux
Q51 - Technologie des aliments pour animaux

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

Agences de financement hors UE : Sofiproteol

Projets sur financement : (FRA) Projet Biopeptide

Auteurs et affiliations

• Beaubier Sophie, Université de Lorraine (FRA) - auteur correspondant
• Durand Erwann, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0002-0306-8081 - auteur correspondant
• Lenclume Charles, Université de Montpellier (FRA)
• Fine Frédéric, Terres Inovia (FRA)
• Aymes Arnaud, CNRS (FRA)
• Framboisier Xavier, Université de Lorraine (FRA)
• Kapel Romain, Université de Lorraine (FRA)
• Villeneuve Pierre, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0003-1685-1494

Source : Cirad-Agritrop (https://agritrop.cirad.fr/604620/)

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