A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria

Brual Typhaine, Effantin Geraldine, Baltenneck Julie, Attaiech Laetitia, Grosbois Cloé, Royer Monique, Cigna Jérémy, Faure Denis, Hugouvieux-Cotte-Pattat Nicole, Gueguen Erwan. 2023. A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria. PLoS Genetics, 19 (4):e1010725, 23 p.

Url - jeu de données - Entrepôt autre : https://figshare.com/articles/figure/Growth_curves_of_i_D_solani_i_strains_and_mutant_derivatives_/22713108

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Anthropologie-Ethnologie

Résumé : The necrotrophic plant pathogenic bacterium Dickeya solani emerged in the potato agrosystem in Europe. All isolated strains of D. solani contain several large polyketide synthase/non-ribosomal peptide synthetase (PKS/NRPS) gene clusters. Analogy with genes described in other bacteria suggests that the clusters ooc and zms are involved in the production of secondary metabolites of the oocydin and zeamine families, respectively. A third cluster named sol was recently shown to produce an antifungal molecule. In this study, we constructed mutants impaired in each of the three secondary metabolite clusters sol, ooc, and zms to compare first the phenotype of the D. solani wild-type strain D s0432-1 with its associated mutants. We demonstrated the antimicrobial functions of these three PKS/NRPS clusters against bacteria, yeasts or fungi. The cluster sol, conserved in several other Dickeya species, produces a secondary metabolite inhibiting yeasts. Phenotyping and comparative genomics of different D. solani wild-type isolates revealed that the small regulatory RNA ArcZ plays a major role in the control of the clusters sol and zms. A single-point mutation, conserved in some Dickeya wild-type strains, including the D. solani type strain IPO 2222, impairs the ArcZ function by affecting its processing into an active form.

Mots-clés Agrovoc : mutation, Saccharomyces cerevisiae, Magnaporthe, levure, métabolite secondaire, antimicrobien, bactéricide, phénotype, Dickeya

Mots-clés libres : Yeast, Saccharomyces cerevisiae, Candida albicans, Fungi, Escherichia coli, Secondary metabolites, Genomics, Ascomycetes

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Agence Nationale de la Recherche

Projets sur financement : (FRA) Etude d'un nouveau type de chaperonne à ARN, (FRA) Identification par Tn-seq de nouveaux déterminants génétiques de bactéries phytopathogènes, impliqués dans l'infection des plantes et la persistance dans l'environnement

Auteurs et affiliations

• Brual Typhaine, Université de Lyon (FRA)
• Effantin Geraldine, CNRS (FRA)
• Baltenneck Julie, Université de Lyon (FRA)
• Attaiech Laetitia, CIRI (FRA)
• Grosbois Cloé, Université de Lyon (FRA)
• Royer Monique, CIRAD-BIOS-UMR PHIM (FRA)
• Cigna Jérémy, French Federation of Seed Potato Growers (FRA)
• Faure Denis, CNRS (FRA)
• Hugouvieux-Cotte-Pattat Nicole, INSA de Lyon (FRA)
• Gueguen Erwan, INSA de Lyon (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/604636/)

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