Glutathione transferases: Surrogate targets for discovering biologically active compounds

Barbier Muriel, Perrot Thomas, Salzet Guillaume, Amusant Nadine, Dumarçay Stéphane, Gérardin Philippe, Morel-Rouhier Mélanie, Sormani Rodnay, Gelhaye Eric. 2020. Glutathione transferases: Surrogate targets for discovering biologically active compounds. Journal of Natural Products, 83 (10) : 2960-2966.

Url - jeu de données - Entrepôt autre : https://figshare.com/collections/Glutathione_Transferases_Surrogate_Targets_for_Discovering_Biologically_Active_Compounds/5139407

Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q2, Sujet : CHEMISTRY, MEDICINAL / Quartile : Q2, Sujet : PHARMACOLOGY & PHARMACY

Résumé : Glutathione transferases comprise a large class of multifunctional enzymes, some involved in detoxification pathways. Since these enzymes are able to interact with potentially toxic molecules, they could be used as targets to screen for compounds with biological activity. To test this hypothesis, glutathione transferases (GSTs) from the white-rot fungus Trametes versicolor have been used to screen for antifungal molecules from a library of tropical wood extracts. The interactions between a set of six GSTs from the omega class and 116 extracts from 21 tropical species were quantified using a high-throughput thermal shift assay. A correlation between these interactions and the antifungal properties of the tested extracts was demonstrated. This approach has been extended to the fractionation of an Andira coriacea extract and led to the detection of maackiain and lapachol in this wood. Altogether, the present results supported the hypothesis that such detoxification enzymes could be used to detect biologically active molecules.

Mots-clés Agrovoc : activité enzymatique, propriété antifongique, détoxification, transférase, enzyme, interactions biologiques, bois, bois tropical

Agences de financement hors UE : Agence Nationale de la Recherche

Projets sur financement : (FRA) Recherches avancées sur l'arbre et les écosytèmes forestiers

Auteurs et affiliations

• Barbier Muriel, Université de Lorraine (FRA)
• Perrot Thomas, Heinrich-Heine Universität (DEU)
• Salzet Guillaume, Université de Lorraine (FRA)
• Amusant Nadine, CIRAD-ES-UMR Ecofog (GUF)
• Dumarçay Stéphane, Université de Lorraine (FRA)
• Gérardin Philippe, Université de Lorraine (FRA)
• Morel-Rouhier Mélanie, Université de Lorraine (FRA)
• Sormani Rodnay, Université de Lorraine (FRA)
• Gelhaye Eric, INRA (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/605394/)

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