Molecular diversity of yam virus Y and identification of banana mild mosaic virus isolates infecting yam (Dioscorea spp.)

Diouf Mame Boucar, Guyader Sébastien, Nopoly Marie-Michelle, Gaspard Olyvia, Filloux Denis, Candresse Thierry, Marais Armelle, Teycheney Pierre-Yves, Umber Marie. 2023. Molecular diversity of yam virus Y and identification of banana mild mosaic virus isolates infecting yam (Dioscorea spp.). Archives of Virology, 168:180, 8 p.

https://doi.org/10.1007/s00705-023-05809-3

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Url - jeu de données - Entrepôt autre : https://doi.org/10.57745/GNUXVA / Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/nuccore/OP495428 / Url - jeu de données - Entrepôt autre : https://www.ncbi.nlm.nih.gov/nuccore/OP495507

Résumé : Two members of the family Betaflexiviridae associated with yam (Dioscorea spp.) have been described so far: yam latent virus (YLV) and yam virus Y (YVY). However, their geographical distribution and molecular diversity remain poorly documented. Using a nested RT-PCR assay, we detected YVY in D. alata, D. bulbifera, D. cayenensis, D. rotundata, and D. trifida in Guadeloupe, and in D. rotundata in Côte d'Ivoire, thus extending the known host range of this virus and geographical distribution. Using amplicon sequencing, we determined that the molecular diversity of YVY in the yam samples analyzed in this work ranged between 0.0 and 29.1% and that this diversity is partially geographically structured. We also identified three isolates of banana mild mosaic virus (BanMMV) infecting D. alata in Guadeloupe, providing the first evidence for BanMMV infection in yam.

Mots-clés Agrovoc : identification, virus des végétaux, Dioscorea, Dioscorea cayenensis, Dioscorea rotundata, distribution géographique, séquence nucléotidique, Betaflexiviridae, phylogénie, Dioscorea alata, indexage des virus des plantes