Discriminating lineages of Batrachochytrium dendrobatidis using quantitative PCR

Ghosh Pria, Verster Ruhan, Sewell Thomas R., O'Hanlon Simon J., Brookes Lola M., Rieux Adrien, Garner Trenton W. J., Weldon Ché, Fisher Matthew C.. 2021. Discriminating lineages of Batrachochytrium dendrobatidis using quantitative PCR. Molecular Ecology Resources, 21 (5) : 1452-1459.

https://doi.org/10.1111/1755-0998.13299

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Url - jeu de données - Entrepôt autre : https://doi.org.10.5061/dryad.r2280gbbb

Quartile : Q1, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY / Quartile : Q1, Sujet : ECOLOGY / Quartile : Q1, Sujet : EVOLUTIONARY BIOLOGY

Résumé : The ability to detect and monitor infectious disease in a phylogenetically informative manner is critical for their management. Phylogenetically informative diagnostic tests enable patterns of pathogen introduction or changes in the distribution of genotypes to be measured, enabling research into the ecology of the pathogen. Batrachochytrium dendrobatidis (Bd), a causative agent of chytridiomycosis in amphibian populations, emerged worldwide in the 21st century and is composed of six lineages which are display varying levels of virulence in their hosts. Research into the distribution, ecology and pathogenicity of these lineages has been hampered by an inability to type lineage efficiently. Here, we describe a lineage-specific TaqMan qPCR assay that differentiates the two lineages of Bd most commonly associated with chytridiomycosis: BdGPL and BdCAPE. We demonstrate how this assay can be used for the surveillance of wild populations of amphibians in Southern Africa using skin swabs, tissue samples and cultured isolates.

Mots-clés Agrovoc : phylogénie, PCR, lignée, adn, maladie fongique, maladie infectieuse

Mots-clés géographiques Agrovoc : Brésil, Afrique du Sud, Afrique

Mots-clés libres : Batrachochytrium dendrobatidis, Chytrid, Diagnostic, Lineage, QPCR

Agences de financement hors UE : Morris Animal Foundation, Leverhulme Trust, Natural Environment Research Council

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