GARP transcription factors repress Arabidopsis nitrogen starvation response via ROS-dependent and -independent pathways

Safi Alaeddine, Medici Anna, Szponarski Wojciech, Martin Florence, Clément-Vidal Anne, Marshall-Colón Amy, Ruffel Sandrine, Gaymard F., Rouached Hatem, Leclercq Julie, Coruzzi Gloria M., Lacombe Benoit, Krouk G.. 2021. GARP transcription factors repress Arabidopsis nitrogen starvation response via ROS-dependent and -independent pathways. Journal of Experimental Botany, 72 (10) : 3881-3901.

Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : Plants need to cope with strong variations of nitrogen availability in the soil. Although many molecular players are being discovered concerning how plants perceive NO3− provision, it is less clear how plants recognize a lack of nitrogen. Following nitrogen removal, plants activate their nitrogen starvation response (NSR), which is characterized by the activation of very high-affinity nitrate transport systems (NRT2.4 and NRT2.5) and other sentinel genes involved in N remobilization such as GDH3. Using a combination of functional genomics via transcription factor perturbation and molecular physiology studies, we show that the transcription factors belonging to the HHO subfamily are important regulators of NSR through two potential mechanisms. First, HHOs directly repress the high-affinity nitrate transporters, NRT2.4 and NRT2.5. hho mutants display increased high-affinity nitrate transport activity, opening up promising perspectives for biotechnological applications. Second, we show that reactive oxygen species (ROS) are important to control NSR in wild-type plants and that HRS1 and HHO1 overexpressors and mutants are affected in their ROS content, defining a potential feed-forward branch of the signaling pathway. Taken together, our results define the relationships of two types of molecular players controlling the NSR, namely ROS and the HHO transcription factors. This work (i) up opens perspectives on a poorly understood nutrient-related signaling pathway and (ii) defines targets for molecular breeding of plants with enhanced NO3− uptake.

Mots-clés Agrovoc : amélioration des plantes, facteur de transcription, régulation physiologique, réponse de la plante, transcription, nitrate, azote assimilable par la plante, génome, Arabidopsis, facteur édaphique, biotechnologie

Mots-clés libres : Cell sorting, GARP transcription factors, Nitrogen starvation response, Root protoplasts, ROS, Target

Agences de financement hors UE : Agence Nationale de la Recherche, Centre National de la Recherche Scientifique

Projets sur financement : (FRA) Identification de régulations moléculaires majeures impliquées dans l’adaptation des plantes à la disponibilité en azote

Auteurs et affiliations

• Safi Alaeddine, Ghent University (BEL) - auteur correspondant
• Medici Anna, Montpellier SupAgro (FRA)
• Szponarski Wojciech, INRAE (FRA)
• Martin Florence, CIRAD-BIOS-UMR AGAP (FRA)
• Clément-Vidal Anne, CIRAD-BIOS-UMR AGAP (FRA)
• Marshall-Colón Amy, University of Illinois (USA)
• Ruffel Sandrine, Université de Montpellier (FRA)
• Gaymard F., INRAE (FRA)
• Rouached Hatem, MSU (USA)
• Leclercq Julie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8110-3762
• Coruzzi Gloria M., State University of New York (USA)
• Lacombe Benoit, CNRS (FRA)
• Krouk G., CNRS (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/605885/)

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