A tell tail sign: A conserved C-terminal tail-anchor domain targets a subset of pathogen effectors to the plant endoplasmic reticulum

Breeze Emily, Vale Victoria, McLellan Hazel, Pecrix Yann, Godiard Laurence, Grant Murray, Frigerio Lorenzo. 2023. A tell tail sign: A conserved C-terminal tail-anchor domain targets a subset of pathogen effectors to the plant endoplasmic reticulum. Journal of Experimental Botany, 74 (10) : 3188-3202.

https://doi.org/10.1093/jxb/erad075

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Résumé : The endoplasmic reticulum (ER) is the entry point to the secretory pathway and, as such, is critical for adaptive responses to biotic stress, when the demand for de novo synthesis of immunity-related proteins and signalling components increases significantly. Successful phytopathogens have evolved an arsenal of small effector proteins which collectively reconfigure multiple host components and signalling pathways to promote virulence; a small, but important, subset of which are targeted to the endomembrane system including the ER. We identified and validated a conserved C-terminal tail-anchor motif in a set of pathogen effectors known to localize to the ER from the oomycetes Hyaloperonospora arabidopsidis and Plasmopara halstedii (downy mildew of Arabidopsis and sunflower, respectively) and used this protein topology to develop a bioinformatic pipeline to identify putative ER-localized effectors within the effectorome of the related oomycete, Phytophthora infestans, the causal agent of potato late blight. Many of the identified P. infestans tail-anchor effectors converged on ER-localized NAC transcription factors, indicating that this family is a critical host target for multiple pathogens.

Mots-clés Agrovoc : relation hôte pathogène, effecteur moléculaire, bioinformatique, Plasmopara halstedii, mitochondrie, Solanum tuberosum, réticulum endoplasmique

Mots-clés libres : Endomembrane, Endoplasmic reticulum (ER), Oomycete effectors, NAC with Transmembrane Motif1-like (NTL), Phytophthora infestans, Tail anchor

Classification Agris : H20 - Maladies des plantes
U60 - Sciences de la vie et de la Terre
F60 - Physiologie et biochimie végétale

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Biotechnology and Biological Sciences Research Council

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