Genetic structure and TALome analysis highlight a high level of diversity in burkinabe Xanthomonas Oryzae pv. oryzae populations

Diallo A., Wonni Issa, Sicard Anne, Blondin Laurence, Gagnevin Lionel, Vernière Christèle, Szurek Boris, Hutin Mathilde. 2023. Genetic structure and TALome analysis highlight a high level of diversity in burkinabe Xanthomonas Oryzae pv. oryzae populations. Rice, 16:33, 14 p.

https://doi.org/10.1186/s12284-023-00648-x

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Résumé : Bacterial Leaf Blight of rice (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a major threat for food security in many rice growing countries including Burkina Faso, where the disease was first reported in the 1980's. In line with the intensification of rice cultivation in West-Africa, BLB incidence has been rising for the last 15 years. West-African strains of Xoo differ from their Asian counterparts as they (i) are genetically distant, (ii) belong to new races and, (iii) contain reduced repertoires of Transcription Activator Like (TAL) effector genes. In order to investigate the evolutionary dynamics of Xoo populations in Burkina Faso, 177 strains were collected from 2003 to 2018 in three regions where BLB is occurring. Multilocus VNTR Analysis (MLVA-14) targeting 10 polymorphic loci discriminated 24 haplotypes and showed that Xoo populations were structured according to their geographical localization and year of collection. Considering their major role in Xoo pathogenicity, we assessed the TAL effector repertoires of the 177 strains upon RFLP-based profiling. Surprisingly, an important diversity was revealed with up to eight different RFLP patterns. Finally, comparing neutral vs. tal effector gene diversity allowed to suggest scenarios underlying the evolutionary dynamics of Xoo populations in Burkina Faso, which is key to rationally guide the deployment of durably resistant rice varieties against BLB in the country.

Mots-clés Agrovoc : Xanthomonas oryzae, génétique des populations, variation génétique, surveillance épidémiologique, Oryza sativa, génotype, dynamique des populations, phylogénie, Xanthomonas, pathotype, épidémiologie, résistance génétique

Mots-clés géographiques Agrovoc : Burkina Faso, France, Mali

Mots-clés libres : Rice, Bacterial leaf blight, Xoo, TALE, Genotyping, Microsatellites, Molecular epidemiology

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Consortium of International Agricultural Research Centers, Ambassade de France au Burkina Faso, Agence Nationale de la Recherche

Projets sur financement : (FRA) MUSE

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