Constitutive proteins of lumpy skin disease virion assessed by next-generation proteomics

Schlosser-Perrin Léo, Holzmuller Philippe, Fernandez Bernard, Miotello Guylaine, Dahmani Noureddine, Neyret Aymeric, Bertagnoli Stéphane, Armengaud Jean, Caufour Philippe. 2023. Constitutive proteins of lumpy skin disease virion assessed by next-generation proteomics. Journal of Virology, 97 (10), 32 p.

https://doi.org/10.1128/jvi.00723-23

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Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Lumpy skin disease (LSD) virus, a ruminant poxvirus of the Capripoxvirus genus, is the etiologic agent of an economically important cattle disease categorized as a notifiable disease by the World Organisation for Animal Health. The large and complex enveloped LSD virus (LSDV) particle encloses a double-stranded linear DNA genome of 151 kbp, comprising 156 predicted ORFs, together with a variety of proteins that are not yet identified. In parallel with a recent widespread expansion of LSD, many LSDV whole genomes have been sequenced, but knowledge on protein composition of the LSDV particle remains missing. In this study, LSD mature virions (MV) from strain KSGP-0240 were purified through a multistep ultracentrifugation process. The protein composition of LSD virions was then analyzed using label-free shotgun proteomics, based upon nano-liquid chromatography (LC) and tandem mass spectrometry. This procedure resulted in the identification of a total of 111 LSDV proteins. Considering that this first MV proteome extended beyond packaged proteins into the field of contaminants, an analytical methodology was developed and made it possible to select 66 viral proteins as candidates for packaging into MV. These viral proteins were analyzed comparatively with proteins previously demonstrated to be constitutive of the vaccinia virus MV particle. Offering for the first time a comprehensive proteomic analysis of an LSDV strain, this study contributes to our understanding of the structural features of infectious LSDV MV particles and paves the way for further systematic proteomic characterization of other LSDV strains.

Mots-clés Agrovoc : capripoxvirus, maladie nodulaire cutanée, protéine, virologie, immunologie, spectrométrie de masse, maladie de la peau

Mots-clés géographiques Agrovoc : France

Mots-clés libres : Lumpy skin disease, Mature virion, Viral particle proteome, Viral proteins, Capripoxvirus

Classification Agris : L73 - Maladies des animaux
U60 - Sciences de la vie et de la Terre

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement hors UE : Ministère de l'Agriculture et de l'Alimentation, Agence Nationale de la Recherche, Région Occitanie Pyrénées-Méditerranée

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