Using enzymes to harvest fatty acids from galactosyldiacylglycerols, the most abundant lipids in plant biomass

Amara Sawsan, Lecomte Jérôme, Barouh Nathalie, Sahaka Moulay, Lafont Dominique, Rodier Jean-David, Parsiegla Goetz, Demarne Frédéric, Gontero Brigitte, Villeneuve Pierre, Carrière Frédéric. 2024. Using enzymes to harvest fatty acids from galactosyldiacylglycerols, the most abundant lipids in plant biomass. ACS Sustainable Chemistry and Engineering, 12 : 4103-4113.

Url - jeu de données - Entrepôt autre : https://figshare.com/collections/Using_Enzymes_to_Harvest_Fatty_Acids_from_Galactosyldiacylglycerols_the_Most_Abundant_Lipids_in_Plant_Biomass/7097569

Résumé : Mono- and digalactosyldiacylglycerols (MGDG, DGDG), the main lipids of plant photosynthetic membranes, represent a large but unexploited reservoir of fatty acids on earth. They are dispersed in plant biomass (milligrams per gram of dry mass) and not as accessible as vegetable oils by simple physical means. The identification and characterization of galactolipid acyl hydrolases, or galactolipases, raise the possibility to use these biocatalysts for the bioconversion of galactolipids. Here, we show that two enzymes of mammalian and microbial origins, pancreatic lipase-related protein 2 from guinea pig (GPLRP2) and cutinase from Fusarium solani, have the capacity to directly and fully release the fatty acids of MGDG and DGDG present in various plant leaves and green wastes. This high substrate accessibility to enzymes was further explored by performing alcoholysis reactions in situ and showing the conversion of galactolipid fatty acids into fatty acid alkyl esters (FAAE) when the enzyme and leaves were incubated in the presence of 6 or 2.5 M ethanol. These findings pave the way to the recovery of fatty acids dispersed in green biomass and the exploitation of an additional and renewable source of fatty acids for oleochemistry and nutrition in a context of competition for vegetable oils.

Mots-clés Agrovoc : activité enzymatique, lipide, acide gras, biocatalyseur, enzyme, déchet organique, hydrolase

Mots-clés libres : Lipids, Enzymatic hydrolysis, Galactolipids, Biocatalysis, Cutinase, Digalactosyldiacylglycerol, Galactolipase, Lipase, Monogalactosyldiacylglycerol

Classification Agris : F60 - Physiologie et biochimie végétale
Q60 - Traitement des produits agricoles non alimentaires
U50 - Sciences physiques et chimie

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

Agences de financement hors UE : Agence Nationale de la Recherche

Projets sur financement : (FRA) Utilisation de galactolipases pour extraire les acides gras de la principale source de lipides présente sur la terre. - Using galactolipases for extracting fatty acids from the main source of lipids on the earth

Auteurs et affiliations

• Amara Sawsan, CNRS (FRA)
• Lecomte Jérôme, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0001-9942-5858
• Barouh Nathalie, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0001-6956-1349
• Sahaka Moulay, Aix-Marseille université (FRA)
• Lafont Dominique, UCLB (FRA)
• Rodier Jean-David, Gattefossé (FRA)
• Parsiegla Goetz, Aix-Marseille université (FRA)
• Demarne Frédéric, Gattefossé (FRA)
• Gontero Brigitte, Aix-Marseille université (FRA)
• Villeneuve Pierre, CIRAD-PERSYST-UMR Qualisud (FRA) ORCID: 0000-0003-1685-1494
• Carrière Frédéric, CNRS (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/608669/)

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