Cytogenomic characterization of transposable elements and satellite DNA in Passiflora L. Species

Santos Silva Gonçalo, Magalhães Souza Margarete, de Carvalho Cayres Pamponét Vanessa, Micheli Fabienne, Ferreira de Melo Cláusio Antônio, Gomes de Oliveira Sarah, Almeida Costa Eduardo. 2024. Cytogenomic characterization of transposable elements and satellite DNA in Passiflora L. Species. Genes, 15 (4):418, 15 p.

htts://doi.org/10.3390/genes15040418

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Résumé : The species Passiflora alata, P. cincinnata, and P. edulis have great economic value due to the use of their fruits for human consumption. In this study, we compared the repetitive genome fractions of these three species. The compositions of the repetitive DNA of these three species' genomes were analyzed using clustering and identification of the repetitive sequences with RepeatExplorer. It was found that repetitive DNA content represents 74.70%, 66.86%, and 62.24% of the genome of P. alata, P. edulis, and P. cincinnata, respectively. LTR Ty3/Gypsy retrotransposons represent the highest genome proportions in P. alata and P. edulis, while Ty1/Copia comprises the largest proportion of P. cincinnata genome. Chromosomal mapping by Fluorescent In Situ Hybridization (FISH) showed that LTR retrotransposons have a dispersed distribution along chromosomes. The subtelomeric region of chromosomes is where 145 bp satellite DNA is located, suggesting that these elements may play important roles in genome structure and organization in these species. In this work, we obtained the first global characterization of the composition of repetitive DNA in Passiflora, showing that an increase in genome size is related to an increase in repetitive DNA, which represents an important evolutionary route for these species.

Mots-clés Agrovoc : Passiflora edulis, transposon, ADN satellite, génome, carte génétique, Passiflora, adn, marqueur génétique

Mots-clés géographiques Agrovoc : Brésil, Bahia

Mots-clés libres : FISH, Passiflora alata, Passiflora cincinnata, Passiflora edulis, Satellite DNA, Transposable elements, Ty1/Copia, Ty3/Gypsy

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Agences de financement hors UE : Fundação de Amparo à Pesquisa do Estado da Bahia, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

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