A voltammetric peptide biosensor for Cu2+ metal ion quantification in coffee seeds

Magarelli Gabriella, Gomes da Silva Jonatas, Luchtenberg Ribeiro Caroline, Viana de Freitas Thiago, Aparecida Rodrigues Magali, de Souza Gil Eric, Marraccini Pierre, Rodrigues de Souza Jurandir, Silva Pires de Castro Clarissa, Porto Bemquerer Marcelo. 2024. A voltammetric peptide biosensor for Cu2+ metal ion quantification in coffee seeds. Journal of Inorganic Biochemistry, 251:112441, 7 p.

https://doi.org/10.1016/j.jinorgbio.2023.112441

Article de revue ; Article de recherche ; Article de revue à facteur d'impact

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Résumé : A prion-derived copper(II)-binding peptide was assembled onto a gold electrode for the building of a voltammetric biosensor for measuring the Cu2+ metal ion in biological samples. The chosen sequence was H-CVNITKQHTVTTTT-NH2, with an appended cysteine residue for binding to the gold surface as a self-assembled monolayer and a histidine residue as the anchorage point for copper(II) complexation. The biosensor showed a linear range of 10−7 to 10−6 M with an 8.0 × 10−8 M detection limit and a 1.0 × 10−7 M quantification limit, with good precision, trueness, and absence of matrix effect. The quantification of Cu2+ was performed in the presence of other transition metal ions, such as Zn2+, Cd2+, Fe2+, or Ni2+, which indicates the excellent selectivity of the biosensor. When the modified electrode was applied for measuring copper(II) in calcined coffee seeds, a difference in copper amount was observed between two Coffea arabica cultivars that were submitted to a treatment with a copper-based antifungal, showing the applicability of the biosensor in the agricultural field.

Mots-clés Agrovoc : Coffea arabica, cuivre, biodétecteur, peptide, technique analytique, technologie alimentaire, propriété technologique, fongicide, Coffea, voltammétrie

Mots-clés libres : Coffea arabica, Cuivre, Coffee seeds, Peptide biosensor, Voltammetry, Prion peptide

Classification Agris : F60 - Physiologie et biochimie végétale
Q04 - Composition des produits alimentaires

Champ stratégique Cirad : CTS 3 (2019-) - Systèmes alimentaires

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