Leaf volatile organic compounds profiles from two citrus genotypes differing in susceptibility to Phytophthora citrophthora infection

Maserti Bianca Elena, Michelozzi Marco, Cencetti Gabriele, Riolo Paolo, La Spada Federico, Aloi Francesco, Pane Antonella, Bartolini Paola, Pecori Francesco, De Andrade Silva Edson Mario, Da Silva Gesteira Abelmon, Micheli Fabienne, Cacciola Santa Olga. 2024. Leaf volatile organic compounds profiles from two citrus genotypes differing in susceptibility to Phytophthora citrophthora infection. Physiological and Molecular Plant Pathology, 133:102319, 9 p.

Résumé : The leaves of two citrus genotypes, citrange 'Carrizo' and sweet orange 'Washington Navel' were artificially infected by the oomycete Phytophthora citrophthora, the causal agent of trunk gummosis and fruit brown rot of citrus, to study the profile of leaf stored terpenoids in the response to the pathogen infection. On 'Washington Navel' leaves the symptoms were more severe and manifested earlier than on 'Carrizo' leaves. Overall, 35 volatile organic compounds (VOCs) were identified by GC-MS analysis in the not-infected (control) and infected leaf extracts from the two citrus genotypes. Interesting the two volatile profiles differed not in the quality, but in the quantity of each compound. After infection, the amount of 15 out of the 35 identified VOCs varied significantly. The level of five metabolites changed in both species, while two of the compounds changed exclusively in 'Citrange' and eight exclusively in 'Washington Navel'. To understand the potential role of differentially expressed stored volatiles in the citrus response mechanisms to the infection, the inhibitory activity of ten of them volatiles was tested in vitro on P. citrophthora. Citral, linalool and trans-2-hexenal completely inhibited the mycelium growth suggesting the possible involvement of these compounds in the defence mechanisms of citrus against P. citrophthora. This study provides new insights into a potential role of stored VOCs in the response mechanisms against the oomycete pathogen P. citrophthora in citrus species. Furthermore, it paves the way for future investigations aimed at assessing the potential utilization of VOCs for priming purposes.

Mots-clés Agrovoc : Citrus, Phytophthora citrophthora, résistance aux maladies, génotype, feuille, orange douce, citrange, composé volatil, agrume, rendement des cultures, gommose, terpénoïde, Phytophthora, stockage

Mots-clés géographiques Agrovoc : Washington

Mots-clés libres : Phytophthora diseases, VOC, GC-MS, Trunk gummosis, Fruit brown rot, Sweet orange, Citrange

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes
Q04 - Composition des produits alimentaires

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agences de financement européennes : European Commission

Agences de financement hors UE : Università di Catania

Programme de financement européen : H2020

Projets sur financement : (ITA) Smart and innovative packaging, postharvest rot management, and shipping of organic citrus fruit, (ITA) PROMETEO «Un village transfrontalier pour protéger les cultures arboricoles méditerranéennes en partageant les connaissances», (EU) SAMOTHRACE

Auteurs et affiliations

• Maserti Bianca Elena, CNR (ITA) - auteur correspondant
• Michelozzi Marco, CNR (ITA)
• Cencetti Gabriele, CNR (ITA)
• Riolo Paolo, University of Catania (ITA)
• La Spada Federico, University of Catania (ITA)
• Aloi Francesco, University of Catania (ITA)
• Pane Antonella, University of Catania (ITA)
• Bartolini Paola, CNR (ITA)
• Pecori Francesco, CNR (ITA)
• De Andrade Silva Edson Mario, UFV (BRA)
• Da Silva Gesteira Abelmon, EMBRAPA (BRA)
• Micheli Fabienne, CIRAD-BIOS-UMR AGAP (FRA)
• Cacciola Santa Olga, University of Catania (ITA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/609627/)

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